Browse

Transcriptome analysis of enterotoxigenic escherichia coli (ETEC) isolates exposed to cabbage
양배추 접촉 장독소형 대장균의 전사체 분석

DC Field Value Language
dc.contributor.advisor유상렬-
dc.contributor.author정승희-
dc.date.accessioned2017-07-14T06:48:19Z-
dc.date.available2017-07-14T06:48:19Z-
dc.date.issued2017-02-
dc.identifier.other000000140869-
dc.identifier.urihttps://hdl.handle.net/10371/125984-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2017. 2. 유상렬.-
dc.description.abstractEnterotoxigenic Escherichia coli (ETEC) is a food-borne pathogen causing diarrhea among children in developing countries as well as travelers in ETEC endemic areas. Recent studies have revealed that many outbreaks of ETEC were mediated by fresh produce such as sprouts or lettuce. To understand how ETEC adapt to fresh produce, ETEC FORC31 isolated from stool of a patient suffered from foodborne disease in Korea was cultivated in the presence of cabbage (Brassica oleracea var. capitata L.). Planktonic cells exposed to cabbage were subjected to RNA sequencing to obtain bacterial gene expression profiles. As a result, among a total of 5,391 annotated coding DNA sequences, 1.13% and 0.74% of total genes were significantly up- or down-regulated when contacted with cabbage for 4 h. Differently expressed genes (p-value ≤ 0.05, fold-change ≥ 5) were grouped mainly into five categories according to COG designations: amino acid transport/metabolism, nucleotide transport/metabolism, cell motility, inorganic ion transport/metabolism, and signal transduction mechanisms. This indicates that ETEC FORC31 may utilize various nutritional factors released from cabbage to promote its growth and survival. Particularly, RNA-Seq revealed that molybdenum-related genes including molybdate transporter and molybdenum cofactor (Moco) biosynthesis operons are differentially expressed when the bacteria were exposed to cabbage. Molybdate used for Moco biosynthesis, and Moco is found in a number of different molybdoenzymes, which involve carbon, sulfur, and nitrogen metabolisms. It was further demonstrated that ETEC FORC31 ΔmoeAB defective in Moco biosynthesis had reduced motility and changed curli production in the presence of excess nitrate. In addition, ΔmoeAB was more sensitive to organic acid than wild type. These results indicate that molybdenum metabolism significantly affects for survival of ETEC in cabbage. Therefore, to reduce ETEC contamination in cabbage, organic acid treatment is carefully conducted because up-regulated Moco biosynthesis genes may affect bacterial acid resistance.-
dc.description.tableofcontentsI. INTRODUCTION 1
II. MATERIALS AND METHODS 5
1. Preparation of cabbage 5
2. Bacterial strains and growth condition 5
3. Construction and complementation of ΔmoeAB mutant 6
4. RNA extraction 8
5. RNA sequencing and analysis of transcriptomes 8
6. Quantitative real-time PCR (qRT-PCR) analysis 9
7. Swimming motility assay 10
8. Assay for curli expression 10
9. Organic acid resistance test 11
III. RESULTS 16
1. Growth of ETEC FORC31 in cabbage 16
2. Gene expression profile of ETEC FORC31 exposed to cabbage 18
3. Gene expression patterns with known function 24
4. qRT-PCR for validation of RNA-sequencing data 37
5. Transcriptional response of genes related to molybdenum metabolism 39
6. Molybdenum cofactor affects motility and curli production of ETEC FORC31 in nitrate supplementation 43
7. Molybdenum cofactor is related to organic acid resistance of ETEC FORC31 47
IV. DISCUSSION 52
V. CONCLUSION 60
VI. REFFERENCES 61
국문 초록 72
-
dc.formatapplication/pdf-
dc.format.extent1264670 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectETEC-
dc.subjectCabbage-
dc.subjectTranscriptome-
dc.subjectRNA-Seq-
dc.subjectMolybdenum-
dc.subjectMolybdoenzyme-
dc.subject.ddc630-
dc.titleTranscriptome analysis of enterotoxigenic escherichia coli (ETEC) isolates exposed to cabbage-
dc.title.alternative양배추 접촉 장독소형 대장균의 전사체 분석-
dc.typeThesis-
dc.contributor.AlternativeAuthorSeunghee Jeong-
dc.description.degreeMaster-
dc.citation.pagesvi,74-
dc.contributor.affiliation농업생명과학대학 농생명공학부-
dc.date.awarded2017-02-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Theses (Master's Degree_농생명공학부)
Files in This Item:
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse