S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Agricultural Biotechnology (농생명공학부) Theses (Master's Degree_농생명공학부)
Anti-obesity Effect of Raphani Semen Extract in Mice Fed a High-fat Diet
고지방식이 마우스에서 무씨추출물의 항비만 효과
- 농업생명과학대학 농생명공학부
- Issue Date
- 서울대학교 대학원
- 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2017. 2. 이기원.
- Raphani Semen, a dried seed of radish, is used as a traditional Asian medicine to treat hypertension and digestive disorders. Sulforaphene (SFEN) is a main bioactive component in Raphani Semen, which has been shown to exhibit anti-adipogenesis effect in vitro. Here SFEN-enriched Raphani Semen extract (RSE) was prepared and effects of RSE on obesity, fatty liver and glucose intolerance were examined in mice fed a high-fat diet (HFD). Male C57BL/6J mice were fed a HFD for 16 weeks with or without RSE or black bean peptide (BBP) (n=9/group). Body weights of HFD-fed mice increased as compared to standard diet (SD), whereas both RSE and BBP suppressed HFD-induced increase in body weight after 16 weeks. In addition, RSE-supplementation inhibited HFD-induced fatty liver in mice by reducing hepatic triglyceride (TG) accumulation. To further investigate the underlying mechanisms involved in the reduction of body weight and liver TG in RES-supplemented mice, the expression levels of fatty acid metabolism related genes in epididymal white adipose tissue (eWAT) and liver were evaluated. HFD significantly decreased the expression levels of pgc1 as compared to SD, but RSE increased their levels in eWAT. HFD also reduced the expression levels of cpt-1a and acox1 in liver, whereas RSE significantly increased the levels of both genes. These data indicate that RSE suppressed HFD-induced obesity and fatty liver via upregulation of fatty acid metabolism. Moreover, RSE supplementation dramatically improved glucose tolerance as compared to HFD-fed mice. These results suggest that RSE can be used to prevent obesity and metabolic syndromes.