S-Space College of Medicine/School of Medicine (의과대학/대학원) Dermatology (피부과학전공) Journal Papers (저널논문_피부과학전공)
H2O2 accumulation by catalase reduction changes MAP kinase signaling in aged human skin in vivo
- Shin, Mi Hee; Rhie, Gi-eun; Kim, Yeon Kyung; Park, Chi-Hyun; Cho, Kwang Hyun; Kim, Kyu Han; Eun, Hee Chul; Chung, Jin Ho
- Issue Date
- Nature Publishing Group
- J Invest Dermatol 125:221-229
- Catalase/*metabolism/pharmacology; Cells, Cultured; Dermis/cytology/*enzymology; Extracellular Signal-Regulated MAP Kinases/metabolism; Fibroblasts/cytology/enzymology; Hydrogen Peroxide/*metabolism; JNK Mitogen-Activated Protein Kinases/metabolism; MAP Kinase Signaling System/drug effects/*physiology; Matrix Metalloproteinase 1/metabolism; Proto-Oncogene Proteins c-jun/metabolism; Skin Aging/*physiology; Transcription Factor AP-1/metabolism; Up-Regulation/physiology; p38 Mitogen-Activated Protein Kinases/metabolism
- To understand the molecular alterations occurring during the aging process, we compared mitogen-activated protein (MAP) kinase activities in the intrinsically aged and photoaged skins in the same individuals. Furthermore, we investigated the molecular events related to MAP kinase changes in intrinsically aged and photoaged skins. We found that extracellular-signal-regulated kinase (ERK) activity in photoaged skin was reduced, and that the activities of c-Jun N-terminal kinase (JNK) and p38 kinase were increased compared with intrinsically aged skin in the same individuals. Phospho-c-Jun levels and activator protein 1 activities in photoaged skin were also higher than in intrinsically aged skin. Moreover, catalase activity was found to be much reduced in primary dermal fibroblasts from photoaged skin, and as a result, H2O2 accumulated more in primary dermal fibroblasts in photoaged skin. In addition, treating primary dermal fibroblasts from photoaged skin with catalase reduced H2O2 levels, reversed aging-dependent MAP kinase changes, and inhibited matrix metalloproteinase (MMP)-1 expression. Our results indicate that the accumulation of reactive oxygen species due to catalase attenuation may be a critical aspect of the MAP kinase signaling changes that may lead to skin aging and photoaging in human skin in vivo. Thus, the induction and regulation of endogenous antioxidant enzymes including catalase may offer a strategy for preventing and treating skin aging.
- 0022-202X (Print)
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