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Anti-noroviral effects of Lactobacillus sp. isolated from Korean population : 한국인에서 순수배양된 유산균의 항노로바이러스 효과 연구

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dc.contributor.advisor고광표-
dc.contributor.authorKo, Na-yun-
dc.date.accessioned2017-07-19T03:11:54Z-
dc.date.available2017-07-19T03:11:54Z-
dc.date.issued2016-08-
dc.identifier.other000000136691-
dc.identifier.urihttps://hdl.handle.net/10371/128254-
dc.description학위논문 (석사)-- 서울대학교 보건대학원 : 환경보건학과 환경보건학전공, 2016. 8. 고광표.-
dc.description.abstractNorovirus gastroenteritis causes large health and economic costs, including at least 200,000 deaths annually. There is a difficulty of improve available vaccination program cause of limited cell culture system and effective treatment and prevention was not available until recently.
Probiotics is live bacteria have potential health benefits on the host. It is known as playing the role of immune modulation in the human intestine. And previous studies said Probiotic bacteria have antiviral effects via various mechanism. In this study, we focused on the antiviral mechanism of the probiotics through activating innate immune response. Probiotics therapy could be an alternative method of antiviral prevention and modulation against norovirus infection.
In this study, we isolated target strains of Lactobacillus spp. successfully in the feces of Korean infants. And we screened the anti-noroviral activity of isolated Lactobacillus strains. Lactobacillus reuteri SNUG50382 (LR50382) significantly inhibited murine norovirus infection in RAW264.7 cells with having highest probiotics potential.
Both onset and resolution of disease symptoms are rapid, suggesting that components of the innate immune response are critical in norovirus control. To clarify the mechanism of norovirus inhibition of probiotics, we investigated gene expression of Interferon (IFN)-signaling components and IFN-inducible antiviral effectors. LR50382 increased IFN-α and IFN-β levels in RAW264.7 cells compared with the control. Gene expression of IFN regulating factor 7 (IRF7) and IFN-inducible antiviral effectors, Mx1 also increased. Overall, these results indicate that LR50382 efficiently inhibits norovirus replication in vitro due to its modulating capacity of the immune response through promoting type I IFNs, which are key regulators in IFN signaling pathway.
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dc.description.tableofcontentsⅠ. Introduction 1

Ⅱ. Materials and Methods 4
2-1. Description of study subjects and fecal samples 4
2-2. Culturing of fecal microbiota 4
2-3. Bacteria identification 5
2-4. Cells and viruses 5
2-5. In vitro assay to examine MNV inhibition by Lactobacillus 6
2-6. Test of Lactobacillus spp. for acid-tolerance 6
2-7. Test of Lactobacillus spp. for tolerance to bile salts 7
2-8. Antibiotic susceptibility 7
2-9. Cytotoxicity assay 8
2-10. Quantitative RT-PCR 8
2-11. Statistical analysis 9

Ⅲ. Results 10
3-1. Isolation of Lactobacillus strains and cell viability 10
3-2. Inhibition of MNV replication by Lactobacillus strains in vitro 12
3-3. Screening of acid-tolerance of Lactobacillus isolates 14
3-4. Screening of bile acid-tolerance of Lactobacillus isolates 14
3-5. The test of the Antibiotic susceptibility of the LR50382 18
3-6. Antiviral activity of LR50382 on Raw264.7 cells 22
3-7. Mechanism of antiviral activity of LR50382 26

Ⅳ. Discussion 31

Ⅴ. Conclusion 37

Ⅵ. Reference 39

국문 초록 44
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dc.formatapplication/pdf-
dc.format.extent979640 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 보건대학원-
dc.subjectLactobacillus-
dc.subjectantiviral effect-
dc.subjectinnate immune response-
dc.subjectNorovirus-
dc.subjectIsolation-
dc.subject.ddc363-
dc.titleAnti-noroviral effects of Lactobacillus sp. isolated from Korean population-
dc.title.alternative한국인에서 순수배양된 유산균의 항노로바이러스 효과 연구-
dc.typeThesis-
dc.contributor.AlternativeAuthor고나윤-
dc.description.degreeMaster-
dc.citation.pages46-
dc.contributor.affiliation보건대학원 환경보건학과-
dc.date.awarded2016-08-
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