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Assigning biofunctionality to polyethylene glycol hydrogel with graphene oxide for the enhancement of cell adhesion and osteogenic differentiation of human adipose-derived stem cells : 인간 지방유래 줄기세포의 세포 부착과 골분화 향상을 위한 폴리에틸렌글리콜 하이드로젤의 그래핀 옥사이드 매개 생체기능성 부여

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Authors

노명경

Advisor
김병수
Major
공과대학 화학생물공학부
Issue Date
2015-08
Publisher
서울대학교 대학원
Keywords
PEGDA hydrogelgraphene oxidehuman adipose-derived stem cellscell adhesionosteogenic differentiation
Description
학위논문 (석사)-- 서울대학교 대학원 : 화학생물공학부, 2015. 8. 김병수.
Abstract
Polyethylene glycol (PEG) hydrogels are attractive biomaterials for stem cell culture due to their tunable material properties and swelling behaviors. However, the lack of cell adhesion sites has been one of the major obstacles to generating functional tissue constructs using PEG hydrogels. To overcome the limitation, we designed graphene oxide (GO)-functionalized polyethylene glycol diacrylate (PEGDA) hydrogels (PEGDA-GO) to assign cell adhesion-dependent biofunctionality. The incorporation of GO into three-dimensional (3D) PEGDA networks improved cell attachment, focal adhesion, and focal adhesion kinase (FAK) signaling activation of encapsulated human adipose-derived stem cells (hADSCs). Compared to unmodified hydrogel, the culture of hADSCs in PEGDA-GO enhanced cell viability and survival. When subsequently cultured under osteoinductive condition, hADSCs in PEGDA-GO underwent in vitro osteogenic differentiation and showed more enhanced osteogenic phenotypes than those in PEGDA counterpart. Regarding that the improvements should be preceded by FAK phosphorylation, the underlying mechanism might consist in GO-mediated focal adhesion. Taken together, GO could serve as an effective biofunctionalizing agent of PEGDA hydrogel for the control of stem cell behaviors and lineage commitment, which could open up new possibilities for the design and creation of functional tissue constructs.
Language
English
URI
https://hdl.handle.net/10371/129389
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