Efficient co-delivery of doxorubicin and gold nanorod in PLGA nanoparticles coated by TNF-a-treated MSC membrane for cancer treatment

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dc.description학위논문 (석사)-- 서울대학교 대학원 : 화학생물공학부, 2016. 8. 김병수.-
dc.description.abstractBone marrow-derived mesenchymal stem cells (MSCs) have been extensively studied for cancer therapy using its homing characteristic to injury and tumor tissues. However, MSCs or MSC-derived exosomes would promote tumor growth. To eliminate this risk and take advantage of MSCs, we separated MSC membranes and coated doxorubicin-gold nanorod-PLGA nanoparticles with the membranes (MMNPs). TNF-α-treated MSCs and endothelial cells near tumor shows upregulated vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) on the cell surface. Since VCAM-1 interacts with VLA-4, TNF-α-treated MMNPs (TNF-α MMNPs) exhibited superior tumor vasculature targeting to the polymeric particles or normal MMNPs. Temperature increase by NIR irradiation caused apoptosis of endothelial cells and adjacent cancer cells, and the nanoparticles flowed into deeper cancer cells leading to death of them.-
dc.description.tableofcontents1. Introduction 6

2. Experimental section 9
2.1 Materials 9
2.2 Cell line 10
2.3 Preparation of DOX-GNR-loaded PLGA Nanoparticles 11
2.4 DOX-GNR-PLGA Nanoparticle Size and Loading Yield 12
2.5 Laser Irradiation and Photothermal Imaging of Nanoparticles 13
2.6 MMNP Synthesis and Characterization 14
2.7 DOX Release of MMNP 16
2.8 MMNP Membrane Protein Characterization 17
2.9 Nanoparticle Endocytosis Analysis 18
2.10 Targeting and Photothermal Effect of MMNP on co-cultured cells 19
2.11 Targeting and Photothermal Effect of MMNP on cancer cells 20

3. Results and Discussion 21
3.1 Synthesis and characterization of DOX-GNR-PLGA nanoparticles 21
3.2 Physicochemical characterization of MMNPs 23
3.3 Endocytosis of MMNPs into HUVEC 26
3.4 Targeting and Photothermal Effect of MMNP 28

4. Conclusions 32

5. References 33

요약 (국문초록) 36
dc.format.extent2161317 bytes-
dc.publisher서울대학교 대학원-
dc.subjectcancer therapy-
dc.subjecttumor vasculature-
dc.subjectcellular membrane-
dc.subjecttargeted drug delivery-
dc.titleEfficient co-delivery of doxorubicin and gold nanorod in PLGA nanoparticles coated by TNF-a-treated MSC membrane for cancer treatment-
dc.contributor.affiliation공과대학 화학생물공학부-
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Theses (Master's Degree_화학생물공학부)
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