S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Chemical and Biological Engineering (화학생물공학부) Theses (Master's Degree_화학생물공학부)
Barium doped Silica-Titania Hollow Nanoparticles embedded Reduced Graphene Oxide Hydrogel as a Scaffold for Neural Tissue Engineering
바륨 도핑 실리카-티타니아 할로우 나노입자가 도입된 환원그래핀 하이드로겔의 신경조직 스캐폴드로의 응용
- Heegyeong Yang
- 공과대학 화학생물공학부
- Issue Date
- 서울대학교 대학원
- Neural tissue engineering; RGO hydrogel; nanoparticle; PC12 cell; differentiation; MAPK pathways
- 학위논문 (석사)-- 서울대학교 대학원 : 화학생물공학부, 2017. 2. 장정식.
- Three-dimensional RGO based hydrogels (RGO hydrogel, HNP-RGO hydrogel, Ba-HNP-RGO hydrogel) were successfully fabricated and applied as the scaffold for PC12 cells. All the hydrogels had suitable porous structure and elasticity for neurons. In vitro studies, three RGO hydrogels were highly biocompatible and bioactive. PC12 cells easily adhered and proliferated more rapidly on the three RGO hydrogels than glass (negative control). Moreover, RGO based hydrogels facilitated the elongation and branching of primary neurite through activating MAPK pathways. Particularly, the RGO component activated p38 and JNK pathways, while the HNPs component activated ERK and p38 pathways among MAPK pathways. These results would provide the possibilities of RGO based hydrogels for neural tissue engineering and improved understanding about how RGO works on neurons.