Indole-3-acetic acid prevents human dental pulp stem cells from hydrogen peroxide-induced oxidative toxicity

Abstract

The purpose of present study was to investigate the effect of Indole-3-acetic acid (IAA) on human dental pulp stem cells (hDPSCs) during hydrogen peroxide (H2O2)-induced oxidative toxicity. To validate this situation, we first assessed the effects of H2O2 on viability in hDPSCs at different concentrations ranging from 1-400 μM. The measurement of viable cells was determined after 24h treatment. To demonstrate the effect of IAA, the experimentation was also conducted same as the H2O2 experiment. Next, we tested the effects of IAA on H2O2-induced cytotoxicity in hDPSCs. To assess the effect of IAA as antioxidant, we tested IAA at various concentrations ranging from 1-400 μM after treatment of H2O2 at 180 μM and the cell cycle was also estimated. As our results, H2O2 at 40 μM did not affect cell viability, whereas the inhibitory effect started at 50 μM H2O2 and maximal performance was at 180 μM for 24 h. On the other hands, the treatment of IAA did not influence on the viability of hDPSCs. TheH2O2-induced cytotoxicity was strikingly inhibited in the presence of IAA and these effects were maximal at 150 μM. By the FACS analysis, the cell cycle in control group was seriously destroyed by H2O2 treatment through whole phase. Moreover, the apoptotic cells in control group were remarkably increased comparing with that in non-treat group. Interestingly, the damaged cell cycle by H2O2-induced cytotoxicity was recovered with the treatment IAA at 150 μM and the apoptotic cells were also decreased. In conclusion, the IAA was able to protect hDPSCs from H2O2-induced cytotoxicity via cell cycle restoration.