Browse

Electrical and Magnetic Control of Nanostructures for the Biochip Applications
나노구조체의 전기적 자기적 조절과 이를 이용한 바이오 칩 분야의 응용에 관한 연구

DC Field Value Language
dc.contributor.advisor홍승훈-
dc.contributor.author이동준-
dc.date.accessioned2017-07-19T09:02:28Z-
dc.date.available2017-07-19T09:02:28Z-
dc.date.issued2014-02-
dc.identifier.other000000017432-
dc.identifier.urihttps://hdl.handle.net/10371/131522-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 생물물리 및 화학생물학과, 2014. 2. 홍승훈.-
dc.description.abstractIn this dissertation, we report ways to modulate nanostructures using electrical and magnetic stimulation and their possible applications in biochip application.
First, we report the development of nano-storage wires (NSWs), which can store chemical species and release them at a desired moment via external electrical stimuli. Here, using the electrodeposition process through an anodized aluminum oxide template, we fabricated multisegmented nanowires composed of a polypyrrole segment containing adenosine triphosphate (ATP) molecules, a ferromagnetic nickel segment, and a conductive gold segment. Upon the application of a negative bias voltage, the NSWs released ATP molecules for the control of motor protein activities. Furthermore, NSWs can be printed onto various substrates including flexible or three-dimensional structured substrates by direct writing or magnetic manipulation strategies to build versatile chemical storage devices. Since our strategy provides a means to store and release chemical species in a controlled manner, it should open up various applications such as drug delivery systems and biochips for the controlled release of chemicals.
Additionally, we report the development of ultra-fast enzyme-linked immunosorbent assay (ELISA) method through the utilization of magnetic capture and release cycle. Our system is largely consisting of two components
-
dc.description.abstractarray of primary antibody labeled nickel patterns on the substrate and superparamgnetic nanoparticles functionalized with secondary antibody. Upon the application of cyclic magnetic field, the superparamgnetic nanoparticles are actively captured and released from the nickel patterns. When the superparamagnetic particles captures antigens present in the solution and deliver them to nickel patterns, interaction between the primary antibody-antigen- secondary antibody prevents the superparamgentic particles being released from the nickel patterns during the capture and release cycle. Through this mechanism, it was possible to detect the presence of antigen within 5 minutes. Since our strategy provides a means to reduce the detection time significantly, it should open up various applications such as development of ultra-fast biosensors and cargo delivery in biochips.-
dc.description.tableofcontentsTable of Contents……………………………………………………………………….3
List of figures……………………………………………………………………………..5
Chapter 1 Introduction…………………………………………………………………..6
1.1 Biochip and Biosensors……………………………………………………………..6
1.2 Application of Nanostructures in Biochip Application……………………………..7
1.3 Motivation…………………………………………………………………………..8
Chapter 2 Nano-Storage Wires: Electrical and Magnetic Control of Nanowires……………………………………………………………………….10
2.1 Introduction………………………………………………………………………..10
2.2 Experimental Procedures………………………………………………………….12
2.3 Characterization of Nano-storage Wires…………………………………………..17
2.4 Controlling the Release of ATP and the Biomotor Activity via Electrical Stimuli.21
2.5 Printable Nano-Storage Devices…………………………………………...…….27
2.6 Summary…………………………………………………………………………..30
Chapter 3 Enhancing the Response of ELISA Assay using Magnetic Capture and Release Cycle: Magnetic Control of Magnetic nanoparticles…………...31
3.1 Introduction…………………………………………………….…………………..31
3.2 Experimental Procedure…………………………………………………………....33
3.3 Magnetic Capture and Release Cycle……………………………………………....37
3.4 Enhancement of the Response Time of ELISA Assay through Magnetic Capture and Release Cycle…………………………………………………………………………….39
3.5 Summary…………………………………………………………………………..43
Chapter 4 Conclusions………………………………………………………….44
Chapter 5 References…………………………………………………………...46
Chapter 6 Abstract in Korean………………………………………………….55
-
dc.formatapplication/pdf-
dc.format.extent1413432 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectnanowire-
dc.subjectpolypyrrole-
dc.subjectcontrolled release-
dc.subjectprintable-
dc.subjectbioenergy storage-
dc.subjectnano-bio interface-
dc.subjectsuperparamagnetic nanoparticles-
dc.subjectdetection time-
dc.subjectenzyme-linked immunosorbent assay (ELISA)-
dc.subjectmagnetic capture and release-
dc.subjectbiochip-
dc.subject.ddc571-
dc.titleElectrical and Magnetic Control of Nanostructures for the Biochip Applications-
dc.title.alternative나노구조체의 전기적 자기적 조절과 이를 이용한 바이오 칩 분야의 응용에 관한 연구-
dc.typeThesis-
dc.description.degreeMaster-
dc.citation.pages57-
dc.contributor.affiliation자연과학대학 생물물리 및 화학생물학과-
dc.date.awarded2014-02-
Appears in Collections:
College of Natural Sciences (자연과학대학)Biophysics and Chemical Biology (생물물리 및 화학생물학과)Theses (Master's Degree_생물물리 및 화학생물학과)
Files in This Item:
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse