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Tumoral LINE-1 hypomethylation is associated with poor survival of patients with intrahepatic cholangiocarcinoma
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 강경훈 | - |
dc.contributor.author | 정서린 | - |
dc.date.accessioned | 2017-07-19T10:08:00Z | - |
dc.date.available | 2017-07-19T10:08:00Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.other | 000000140648 | - |
dc.identifier.uri | https://hdl.handle.net/10371/132314 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 종양생물학전공, 2017. 2. 강경훈. | - |
dc.description.abstract | DNA methylation changes occurring in cancer cells are characterized by both promoter CpG island hypermethylation and diffuse genomic hypomethylation. LINE-1 is half-million times repeated in the human genome in an interspersed manner and CpG sites located in 5 untranslated region of LINE-1 are heavily methylated in normal cells and undergo demethylation in association with cancerization. However, little information is available regarding LINE-1 hypomethylation and its prognostic implication in intrahepatic cholangiocarcinomas. We analyzed 172 cases of intrahepatic cholangiocarcinomas for their methylation status at four CpG sites of LINE-1 using pyrosequencing and correlated LINE-1 methylation level with clinicopathological features. Tumor differentiation, lymphatic invasion, and T stage were associated with a low average methylation level of LINE-1 at the four CpG sites | - |
dc.description.abstract | LINE-1 methylation level tended to be lower in high grade differentiation, lymphatic emboli, and higher T stage. Lower methylation status of LINE-1 was significantly associated with lower overall survival in patients with intrahepatic cholangiocarcinoma and found to be an independent prognostic parameter. Our findings suggest that tumoral LINE-1 hypomethylation could be a molecular biomarker heralding poor prognosis of patients with intrahepatic cholangiocarcinoma. A further study is required to validate our findings. | - |
dc.description.tableofcontents | Abstract ⅰ
Contents ⅱ List of Tables ⅳ List of Figures ⅴ Introduction 1 Materials and Methods 3 DNA extraction and bisulfite modification 3 LINE-1 PCR and Pyrosequencing 4 Statistical analysis 5 Results 6 Demographic and clinicopathological data 6 Relationship between LINE-1 methylation level and clinicopathological features 6 Relationship between tumoral LINE-1 methylation status and overall survival of patients with ICC 10 Discussion 15 References 20 Abstract in Korean 24 | - |
dc.format | application/pdf | - |
dc.format.extent | 328626 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | ko | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Cholangiocarcinoma | - |
dc.subject | LINE-1 | - |
dc.subject | methylation | - |
dc.subject | prognosis | - |
dc.subject | pyrosequencing | - |
dc.subject.ddc | 616 | - |
dc.title | Tumoral LINE-1 hypomethylation is associated with poor survival of patients with intrahepatic cholangiocarcinoma | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | 24 | - |
dc.contributor.affiliation | 의과대학 협동과정 종양생물학전공 | - |
dc.date.awarded | 2017-02 | - |
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