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Study of amine-functionalized and angular-shaped iron oxide nanoparticles for MR/fluorescence bimodal imaging applications : 자기공명영상과 형광영상의 동시 획득을 위한 각진 모양과 아민 작용기를 함께 가지는 산화철 나노입자의 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 박원철 | - |
dc.contributor.author | 유동건 | - |
dc.date.accessioned | 2017-07-19T10:58:30Z | - |
dc.date.available | 2017-07-19T10:58:30Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.other | 000000142504 | - |
dc.identifier.uri | https://hdl.handle.net/10371/133238 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 융합과학부, 2017. 2. 박원철. | - |
dc.description.abstract | A simple preparation of water well dispersible angular-shaped and amine-functionalized super-paramagnetic iron oxide nanoparticles (A-SPIONs) was reported in this paper. A-SPIONs was synthesized via heating iron (Ⅲ) acetylacetonate in a mixed solvents of polyethyleneglycol (PEG) and branched polyetheyleneimine (b-PEI) with vigorous stirring. Both PEG and b-PEI provided high water dispersibility by competitive surface coating of A-SPIONs. In addition, b-PEI controlled overall morphologies of A-SPIONs to produce polyhedral nanocrystals with the aid of added halide ions and supplied active amine ends. Due to the amine functional group arising from b-PEI, the A-SPIONs were proved to have both positively charged surface (+29.1mV) and active sites which enable facile functionalization. By using A-SPIONs of 9.42 ± 2.93 nm (TEM observation), a high saturation magnetization value of 75.61 emu/g was measured using superconducting quantum interface device (SQUID). The A-SPIONs sustained stable dispersion in aqueous media with various pH, and their hydrodynamic size was about 13.97 nm in 0.10 M NaCl solution. Through MTT assay, the A-SPIONs were proved to have negligible cellular toxicity in SKOV-3, U87-MG and U251 cell lines. The angular-shaped iron oxide nanoparticles also exhibited high relaxivity for magnetic resonance imaging (MRI) originated from the high magnetization. Cy 5.5 dye-functionalized A-SPIONs (Cy5.5@A-SPIONs) were prepared and serial experiments were conducted to investigate fluorescence imaging applications. According to these results, the A-SPIONs are expected to have potentials in bimodal imaging application. | - |
dc.description.tableofcontents | Introduction 1
Experimental process 7 Materials 7 Characterization 8 MR phantom test 10 Synthesis of USPIO (Ultra small PEGylated iron oxide nanoparticles) 11 Synthesis of the A-SPIONs with different weight ratio of PEG and b-PEI 13 Synthesis of the angular shaped IONPs with potassium bromide 14 Synthesis of the angular shaped IONPs with sodium chloride 15 Synthesis of Cy 5.5@A-SPIONs 16 Cellular viability test 17 Results and discussion 19 Synthesis of angular-shaped superparamagnetic iron oxide nanoparticles (A-SPIONs) 19 Magnetization study of A-SPIONs 34 Aqueous dispersion stability test of A-SPIONs in various pH and NaCl concentration 37 Fluorescence functionalization of the A-SPIONs 41 In vitro cytotoxicity test 44 MR phantom imaging 46 Conclusion 49 References 51 요약 (국문초록) 58 | - |
dc.format | application/pdf | - |
dc.format.extent | 2981900 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Iron oxide nanoparticles | - |
dc.subject.ddc | 620 | - |
dc.title | Study of amine-functionalized and angular-shaped iron oxide nanoparticles for MR/fluorescence bimodal imaging applications | - |
dc.title.alternative | 자기공명영상과 형광영상의 동시 획득을 위한 각진 모양과 아민 작용기를 함께 가지는 산화철 나노입자의 연구 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | 60 | - |
dc.contributor.affiliation | 융합과학기술대학원 융합과학부 | - |
dc.date.awarded | 2017-02 | - |
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