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Surface-modified graphene nanosheets for anticancer drug delivery

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dc.contributor.advisor이동수, 오유경-
dc.contributor.author한정훈-
dc.date.accessioned2017-07-19T11:07:45Z-
dc.date.available2017-07-19T11:07:45Z-
dc.date.issued2015-08-
dc.identifier.other000000056931-
dc.identifier.urihttps://hdl.handle.net/10371/133374-
dc.description학위논문 (석사)-- 서울대학교 융합과학기술대학원 : 분자의학 및 바이오제약학과, 2015. 8. 이동수.-
dc.description.abstractHere, we report reduced graphene oxide (rGO) nanosheets coated with an anti-angiogenic anticancer taurocholate derivative of low-molecular-weight heparin (LHT7) as a tumor-targeting nanodelivery platform for anticancer drugs. Surface coating of LHT7 onto rGO was confirmed using fluorescein isothiocyanate-labeled LHT7, monitored as fluorescence quenching due to associated rGO. Unlike plain rGO, LHT7-coated rGO (LHT-rGO) nanosheets maintained a stable dispersion under physiological conditions for at least 24 h. Moreover, LHT-rGO provided greater loading capacity for doxorubicin (Dox) compared with uncoated rGO nanosheets. Following intravenous administration into KB tumor-bearing mice, in vivo tumor accumulation of LHT-rGO/Dox was 7-fold higher than that of rGO/Dox 24 h post dosing. Intravenously administered LHT-rGO/Dox showed the greatest anti-tumor effect in KB-bearing mice, reducing tumor volume by 92.5% ± 3.1% compared to the untreated group 25 days after tumor inoculation. TUNEL assays revealed that the population of apoptotic cells was highest in the group treated with LHT-rGO/Dox. Taken together, our results demonstrate that LHT-rGO nanosheets confer improved dispersion stability, tumor distribution and in vivo antitumor effects, and may be further developed as a potential active nanoplatform of various anticancer drugs.-
dc.description.tableofcontents1. Introduction
2. Materials and methods
2. 1. Synthesis of LHT7 and F-LHT7
2. 2. Preparation of rGO nanosheets
2. 3. Preparation and characterization of LHT-rGO nanosheets
2. 4. Stability test of LHT-rGO nanosheets
2. 5. Preparation of Dox-loaded nanosheets
2. 6. Cellular uptake test of Dox delivered by LHT-rGO nanosheets
2. 7. In vitro antitumor efficacy study of Dox-loaded nanosheets
2. 8. In vivo molecular imaging
2. 9. In vivo antitumor activity test
2. 10. Statistics
3. Results
3. 1. Characterization of LHT-rGO nanosheets
3. 2. Dispersion stability of LHT-rGO nanosheets
3. 3. Loading of Dox onto LHT-rGO nanosheets
3. 4. Cellular uptake and anticancer efficacy of Dox delivered on LHT-rGO nanosheets
3. 5. In vivo tumor tissue distribution of LHT-rGO/Dox
3. 6. In vivo antitumor effects of LHT-rGO/Dox
4. Discussion
5. Conclusion
References
국문초록
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dc.formatapplication/pdf-
dc.format.extent1358396 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 융합과학기술대학원-
dc.subjectanti-angiogenic ancticancer low molecular weight heparin derivative-
dc.subjectredued graphene oxide-
dc.subjectdispersion stability-
dc.subjectdoxorubicin-
dc.subjectanticancer effect-
dc.subject.ddc610-
dc.titleSurface-modified graphene nanosheets for anticancer drug delivery-
dc.typeThesis-
dc.contributor.AlternativeAuthorHan Jeonghoon-
dc.description.degreeMaster-
dc.citation.pagesv, 33-
dc.contributor.affiliation융합과학기술대학원 분자의학 및 바이오제약학과-
dc.date.awarded2015-08-
Appears in Collections:
Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Theses (Master's Degree_분자의학 및 바이오제약학과)
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