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Pro-inflammatory Function of Secreted Threonyl-tRNA synthetase

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dc.contributor.advisor김성훈-
dc.contributor.author김해준-
dc.date.accessioned2017-07-19T11:08:36Z-
dc.date.available2019-04-17-
dc.date.issued2016-02-
dc.identifier.other000000132485-
dc.identifier.urihttps://hdl.handle.net/10371/133390-
dc.description학위논문 (석사)-- 서울대학교 융합과학기술대학원 : 융합과학기술대학원 분자의학 및 바이오제약학과 의약생명과학 전공, 2016. 2. 김성훈.-
dc.description.abstractARS is an enzyme that pairs tRNA with each amino acid of its own. Among the 20 different amino acids eukaryotes carry, threonyl-tRNA synthetase (TRS) delivers Threonine to tRNA to regulate protein synthesis. However, there is not much of a study done on functions of TRS other than conventional features. It is currently known that TRS is secreted by the external stimulus such as VEGF and TNF. This mechanism causes metastasis and angiogenesis of tumor, but it is not well studied how secreted TRS functions in this circumstances. Inflammatory cytokines are activated by extracellular signaling and then activated cytokines are secreted through the cell membrane. I expected secreted TRS can do a role of inflammatory function. So, I expressed and purified recombinant human TRS in E. coli. Responses of TRS were studied with the purified TRS on various cell lines. Consequently, I found that recombinant TRS can stimulate important cytokines and chemokines such as IL-6 and IL-8, respectively. On this result, for confirming cytokine activities of TRS, TRS plays an important role in inducing the inflammatory immune response. The present study suggested that TRS can be developed as a novel biomarker targeting newly discovered immune responses and inflammatory diseases.-
dc.description.tableofcontentsIntroduction 5

Abbreviations list 7

Material and methods 8
1. Cell culture 8
2. Recombinant human Threonyl-tRNA synthetase production 8
3. Western blotting 9
4. Cytokine assays 9
5. Bioassay involving primary human and mouse cells 10

Results 11
Expression and purification of recombinant human threonyl-tRNA synthetase 11
The second step purification of TRS with anion exchange chromatography 11
A distinct biological activity of TRS in THP1 cells 12
TRS leads biological activation in various type of cell line 12
TRS induces IL-6 in primary cells 12
TRS inducing cytokines in mouse cells lines 13
TRS stimulates inflammatory cytokines productions in primary mouse cells 13
TRS stimulates inflammatory cytokines in mouse organs 14
Full length TRS is required for cytokine activity 14
TRS stimulated signal pathways in THP1 15

Discussion 29

References 31

요약 (국문초록) 34
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dc.formatapplication/pdf-
dc.format.extent1232597 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 융합과학기술대학원-
dc.subjectthreonyl-trna synthetase-
dc.subjectinflammatory cytokine-
dc.subject.ddc610-
dc.titlePro-inflammatory Function of Secreted Threonyl-tRNA synthetase-
dc.typeThesis-
dc.description.degreeMaster-
dc.citation.pages35-
dc.contributor.affiliation융합과학기술대학원 분자의학 및 바이오제약학과-
dc.date.awarded2016-02-
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