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The combinational inhibitory effects of Lactobacillus, 1-monoglycerides, and grapefruit seed extract against Gardnerella vaginalis KCTC 5097
가드넬라(Gardnella vaginalis)균에 대한 유산균, 모노글리세리드와 자몽씨추출물의 병용효과

DC Field Value Language
dc.contributor.advisor지근억-
dc.contributor.author장상단-
dc.date.accessioned2017-07-19T11:56:54Z-
dc.date.available2017-07-19T11:56:54Z-
dc.date.issued2015-02-
dc.identifier.other000000025174-
dc.identifier.urihttps://hdl.handle.net/10371/133944-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 식품영양학과, 2015. 2. 지근억.-
dc.description.abstractThe objective of this study was to observe the antibacterial effects of lactobacilli, 1-monoglycerides (MGs) and grapefruit seed extract (GSE) against the vaginosis-associated pathogen Gardnerella vaginalis. Five lactobacilli were selected from 26 lactobacilli by observing the effect of pH (pH=7) adjusted lactobacilli cell-free culture supernatant (CFCS) on the viability of G. vaginalis. Among them, 3 strains of Lactobacillus showed relatively high inhibition on the growth of G. vaginalis KCTC 5097 during the co-culture of Lactobacillus and G. vaginalis. In addition, the effects of MGs and GSE on the bacterial growth curve were monitored at various levels. Glycerol monolaurate (GML) and GSE had MIC (minimum inhibitory concentration) values of 78.125 ug/mL and 20 ppm, respectively, against G. vaginalis at which the growth of the selected 3 Lactobacillus strains was inhibited to a much lesser degree. In the co-culture assay of Lactobacillus and G. vaginalis in the presence of GML and GSE, the growth of G. vaginalis was considerably suppressed whereas that of Lactobacillus was minimally affected. Taken together, the selected Lactobacillus, GML and GSE could be potential therapeutic candidates to suppress the colonization of G. vaginalis in the vaginal niche.-
dc.description.tableofcontentsContents

Abstract і
Contents ⅲ
List of Figures ⅵ
List of Tables ⅶ
List of Abbreviations viii

1. Introduction 1

2. Materials and methods 4
2.1. Microorganisms and culture conditions 4
2.2. Chemicals 4
2.3. Methods 4
2.3.1. Effect of lactobacilli cell-free culture supernatant(CFCS) on the viability of Gardnerella vaginalis(G. vaginalis) 5
2.3.2. Inhibitory activity of selected lactobacilli on the growth of G. vaginal in the co-culture assay 5
2.3.3. Inhibitory activity of 1- monoglycerides(MGs) and grapefruit seed extract(GSE) on the growth of G. vaginalis 6
2.3.3.1. Preparation of antimicrobial solutions 6
2.3.3.2. Determination of MICs 6
2.3.3.3. Assessment of synergy between glycerol monolaurate(GML) and GSE 7
2.3.4. Co-culture assay of Lactobacillus and G. vaginalis in the presence of GML and GSE 9
2.3.4.1. Sample preparation 9
2.3.4.2. qPCR assays 9

3. Data analysis 11

4. Results 12
4.1. Effect of lactobacilli CFCS on the viability of G. vaginalis 12
4.2. Inhibitory activity of selected lactobacilli on the growth of G. vaginalis in the co-culture assay 15
4.3. Determination of MICs 17
4.4. Combinational effect of GML and GSE on the growth of G. vaginalis 20
4.5. Co-culture assay of Lactobacillus and G. vaginalis in the presence of GML and GSE 25

5. Discussion 28

6. Conclusion 32

References 33

Abstract in Korean 42
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dc.formatapplication/pdf-
dc.format.extent704362 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectAntimicrobial-
dc.subjectCo-culture-
dc.subjectBacterial vaginosis-
dc.subjectLactobacillus-
dc.subjectG. vaginalis-
dc.subject.ddc641-
dc.titleThe combinational inhibitory effects of Lactobacillus, 1-monoglycerides, and grapefruit seed extract against Gardnerella vaginalis KCTC 5097-
dc.title.alternative가드넬라(Gardnella vaginalis)균에 대한 유산균, 모노글리세리드와 자몽씨추출물의 병용효과-
dc.typeThesis-
dc.contributor.AlternativeAuthorShangdan Zhang-
dc.description.degreeMaster-
dc.citation.pages43-
dc.contributor.affiliation생활과학대학 식품영양학과-
dc.date.awarded2015-02-
Appears in Collections:
College of Human Ecology (생활과학대학)Dept. of Food and Nutrition (식품영양학과)Theses (Master's Degree_식품영양학과)
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