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Protective Effects of 5-(3,4-Dihydroxylphenyl)-γ-valerolactone, a major Metabolite of Cocoa Procyanidins, on Hydrogen Peroxide-induced Apoptosis in Primary Cortical Neurons
DHPV의 산화적스트레스에 의한 신경세포사멸 억제 및 항산화 효능

DC Field Value Language
dc.contributor.advisor이기원-
dc.contributor.author김태연-
dc.date.accessioned2017-10-31T07:52:11Z-
dc.date.available2019-04-18-
dc.date.issued2017-08-
dc.identifier.other000000146214-
dc.identifier.urihttps://hdl.handle.net/10371/137587-
dc.description학위논문 (석사)-- 서울대학교 대학원 농업생명과학대학 농생명공학부(바이오모듈레이션전공), 2017. 8. 이기원.-
dc.description.abstractProcyanidins are the most abundant phytochemicals in the dietary food and responsible for the health effects of cocoa. However due to low absorption of procyanidins, recently researchers are focusing on the simple metabolites of cocoa that are catalyzed by intestinal microbiota and exert health effects. The aim of this study is to figure out the neuroprotective effect of 5-(3,4-Dihydroxyphenyl)-γ-valerolactone (DHPV), a min metabolite of cocoa procyanidins, against hydrogen peroxide-induced oxidative stress in primary cortical neurons. Oxidative stress is strongly associated with many diseases such as neurodegenerative disorders, cancers and cardiovascular diseases and oxidative stress is induced by reactive oxygen species such as hydrogen peroxide (H2O2).
In this study, I identified the protective effects of DHPV on oxidative neuronal death. Results showed that H2O2-induced nuclear condensation in apoptotic neurons was inhibited by pre-treatment with DHPV. Pre-treatment with DHPV prevented the H2O2-induced decrease of anti-apoptotic protein, Bcl-2 and inhibited H2O2-induced cleavage of caspase-3 and poly(ADP-ribose) polymerase. I also found that DHPV induced the expression of NADPH:quinone oxidoreductase 1 (NQO1) and enhanced nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2), a principal antioxidant transcription factor in neuronal cells, demonstrating that DHPV protects neurons from H2O2-induced apoptosis by up-regulation of phaseⅡ antioxidant enzymes.
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dc.description.tableofcontentsⅠ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 6
1. Chemicals and reagents 6
2. Primary neuronal culture 7
3. MTT assay 8
4. Tunel assay 9
5. Western blot analysis 10
6. Measurement of intracellular ROS level 12
7. Immunofluorescence 13
8. Statistical analysis 14

Ⅲ. RESULTS 15
1. DHPV concentration up to 80 M has no toxic effect on primary cortical neurons 15
2. DHPV inhibits H2O2-induced apoptosis in primary cortical neurons. 18
3. DHPV attenuates H2O2-induced down-regulation of Bcl-2 and cleavage of caspase-3 and pro-PARP 22
4. DHPV reduces intracellular ROS level by up-regulating the expression of NQO1 26
5. DHPV up-regulate the activation of the major antioxidant transcriptional factor, Nrf2 30
Ⅳ. DISCUSSION 33
Ⅴ. REFERENCES 38
Ⅵ. 국문 초록 43
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dc.formatapplication/pdf-
dc.format.extent877187 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectCocoa procyanidins-
dc.subjectDHPV-
dc.subjectMicrobial metabolite-
dc.subjectApoptosis-
dc.subjectPrimary cortical neurons-
dc.subjectoxidative stress-
dc.subjectH2O2-
dc.subjectNQO1-
dc.subjectNrf2-
dc.subject.ddc571-
dc.titleProtective Effects of 5-(3,4-Dihydroxylphenyl)-γ-valerolactone, a major Metabolite of Cocoa Procyanidins, on Hydrogen Peroxide-induced Apoptosis in Primary Cortical Neurons-
dc.title.alternativeDHPV의 산화적스트레스에 의한 신경세포사멸 억제 및 항산화 효능-
dc.typeThesis-
dc.contributor.AlternativeAuthorKim, Tae Yeon-
dc.description.degreeMaster-
dc.contributor.affiliation농업생명과학대학 농생명공학부(바이오모듈레이션전공)-
dc.date.awarded2017-08-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Biomodulation (바이오모듈레이션전공)Theses (Master's Degree_바이오모듈레이션전공)
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