Preparation of High Valence Silver Complex Nanoparticles for Diabetic Foot Ulcers Application

Abstract

Diabetic foot ulcers(DFUs) is one of the main complications of diabetes mellitus, which seriously affects the health of diabetic patients. Wound healing is an essential process in the treatment of DFUs. The inflammatory with the infection of pathogenic bacteria is one of the important dynamic phases in wound healing. Antibiotics has found a widely utilization in DFUs. Due to antibiotic resistance, silver containing antimicrobial agents deserve greater attention and appear to be effective. To address this problem, we designed and synthesized the high valence silver complex nanoparticles using reverse microemulsion technique for its higher antibacterial ability. Proliferation is another essential step in the wound healing. Wound healing increases the requirement for amino acids for repair and cell growth. L-Phenylalanine is one of the essential amino acids, which means it cannot be synthesized in the human body and must be supplemented by diet. In this study, we conjugate propamidine with Boc-L-phenylalanine which contain L-phenylalanine moiety as a ligand for the silver complex synthesis. Thus, this compound possesses the proliferative potential therapeutic ability due to containing amino acid moiety. Silver(II)-Boc-propamidine nanoparticles have been characterized by spectroscopic techniques. Up to now, we investigated the antibacterial activity of silver(II)-Boc-propamidine nanoparticle against pathogens of DFUs. The newly synthesized high valence silver complex nanoparticles demonstrate better antibacterial activity compared to silver nanoparticles (AgNPs). This study concludes the synthesized silver(II)-Boc-propamidine nanoparticles show better antibacterial ability. And it may serve as next generation therapeutic agent for the treatment of diabetic foot ulcers.