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Studies on the transcriptional regulation of Lefty1 during neuronal differentiation of P19 embryonic carcinoma cells
P19 배아줄기암세포가 신경세포 분화하는 과정에서 Lefty1 유전자의 전사 조절 기전 연구

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dc.contributor.advisor이건수-
dc.contributor.authorEunsong Choi-
dc.date.accessioned2017-10-31T08:32:33Z-
dc.date.available2017-10-31T08:32:33Z-
dc.date.issued2017-08-
dc.identifier.other000000145014-
dc.identifier.urihttps://hdl.handle.net/10371/138077-
dc.description학위논문 (석사)-- 서울대학교 대학원 자연과학대학 생명과학부, 2017. 8. 이건수.-
dc.description.abstractStem cell division should be tightly controlled for a proper balance between self-renewal and differentiation. It is recently known that some types of stem cells have to undergo minimal rounds of cell division in order to turn on expression of a set of genes for differentiation. Here, I searched for genes of which the expression is controlled in a cell-division-dependent manner during neuronal differentiation of P19 embryonic carcinoma cells. I identified three candidate genes, Lefty1, Msi2, and Prtg of which the expressions were upregulated during the retinoic acid-induced neurogenesis in P19 cells. Their expressions were induced by RA in a delayed manner, and such induction was significantly dampened by cell cycle blocking drugs such as thymidine and RO3306. I defined a domain within the Lefty1 promoter sequence that is responsible for cell-division-dependent activation. These results provide a clue on mechanisms how gene expression is controlled by cell division for neuronal differentiation.-
dc.description.tableofcontentsINTRODUCTION 1
MATERIALS AND METHODS 4
Cell culture and synchronization 4
Fluorescence-activated cell sorting 4
Quantitative real-time PCR 4
Luciferase assay 5
Western blot 5
Transcription factor binding site prediction 6
RESULTS 7
Cell division control of P19 cells 7
Neuronal differentiation of P19 cells is induced by retinoic acid 7
Identification of candidate genes with cell-division-dependent expression upon RA-treated P19 cells 8
Definition of the Lefty1 promoter sequence for the cell-division-dependent activation 12
Candidate transcription factors for the cell-division-dependent activation of Lefty1 transcription 12
DISCUSSION 25
REFERENCES 28
ABSTRACT IN KOREAN 31
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dc.formatapplication/pdf-
dc.format.extent1255589 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectCell division-
dc.subjectP19 cell-
dc.subjectNeurogenesis-
dc.subjectDifferentiation-
dc.subjectTranscription-
dc.subject.ddc570-
dc.titleStudies on the transcriptional regulation of Lefty1 during neuronal differentiation of P19 embryonic carcinoma cells-
dc.title.alternativeP19 배아줄기암세포가 신경세포 분화하는 과정에서 Lefty1 유전자의 전사 조절 기전 연구-
dc.typeThesis-
dc.contributor.AlternativeAuthor최은송-
dc.description.degreeMaster-
dc.contributor.affiliation자연과학대학 생명과학부-
dc.date.awarded2017-08-
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Theses (Master's Degree_생명과학부)
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