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Peripheral blood CD4+ and CD8+ T cell type 1 and type 2 cytokine production in atopic asthmatic and normal subjects
DC Field | Value | Language |
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dc.contributor.author | Cho, S-H | - |
dc.contributor.author | Stanciu, L A | - |
dc.contributor.author | Begishivili, T | - |
dc.contributor.author | Bates, P J | - |
dc.contributor.author | Holgate, S T | - |
dc.contributor.author | Johnston, S L | - |
dc.date.accessioned | 2009-11-23T06:04:19Z | - |
dc.date.available | 2009-11-23T06:04:19Z | - |
dc.date.issued | 2002-04-10 | - |
dc.identifier.citation | Clin Exp Allergy. 2002 Mar;32(3):427-33. | en |
dc.identifier.issn | 0954-7894 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11940074 | - |
dc.identifier.uri | https://hdl.handle.net/10371/13819 | - |
dc.description.abstract | BACKGROUND: Increased production of IL-4 and IL-5 and decreased production of IFN-gamma by CD4+ T cells has been implicated in asthma pathogenesis. However, CD8+ T cells also produce type 1 and type 2 cytokines and the relative roles of CD4+ and CD8+ T cell cytokine production in asthma have not been previously studied. OBJECTIVE: To determine the production of the type 1 and type 2 cytokines by CD4+ and CD8+ T cell subsets in asthmatic and normal subjects. METHODS: Intracellular cytokine staining for IL-4, -5, -10, -13 and IFN-gamma was analysed in peripheral blood CD4+ and CD8+ T cells from 24 atopic asthmatic and 20 normal subjects. RESULTS: Both subsets of T cells produced all cytokines studied and there were no significant differences between CD4+ and CD8+ T cells in their capacity to produce either type 1 or type 2 cytokines. There were significantly increased frequencies of IFN-gamma-positive CD4+ (13.1 +/- 2.4%, vs. 7.3 +/- 1.4%) and CD8+ (20.0 +/- 2.9%, vs. 9.6 +/- 2.1%) T cells in asthmatic subjects compared with normal subjects (P < 0.05), but not in frequencies of CD4+ or CD8+ T cells staining positively for IL-4, -5, -10 or -13. CONCLUSION: The frequencies of peripheral blood CD8+ T cells producing type 1 and type 2 cytokines are comparable with the frequencies of CD4+ T cells. There was an increased frequency of IFN-gamma producing CD4+ and CD8+ T cells in asthmatic compared with normal subjects. Further studies investigating T cells derived from the airways and investigating various stages within the disease process are required to further elucidate the importance of type 2 and type 1 T cell cytokine production in the pathogenesis of human allergic disease. | en |
dc.language.iso | en | - |
dc.publisher | Wiley-Blackwell | en |
dc.subject | Adult | en |
dc.subject | Asthma/*blood/*complications | en |
dc.subject | CD4-Positive T-Lymphocytes/*metabolism | en |
dc.subject | CD8-Positive T-Lymphocytes/*metabolism | en |
dc.subject | Cytokines/*biosynthesis/*blood | en |
dc.subject | Forced Expiratory Volume/physiology | en |
dc.subject | Humans | en |
dc.subject | Hypersensitivity, Immediate/*blood/*complications | en |
dc.subject | Immunoglobulin E/blood | en |
dc.subject | Predictive Value of Tests | en |
dc.subject | Reference Values | en |
dc.subject | Severity of Illness Index | en |
dc.subject | Staining and Labeling | en |
dc.subject | Statistics as Topic | en |
dc.subject | T-Lymphocyte Subsets/metabolism | en |
dc.title | Peripheral blood CD4+ and CD8+ T cell type 1 and type 2 cytokine production in atopic asthmatic and normal subjects | en |
dc.type | Article | en |
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