Publications

Detailed Information

Restoration of miR-29b exerts anti-cancer effects on glioblastoma

DC Field Value Language
dc.contributor.authorShin, Jaekyung-
dc.contributor.authorShim, Hyun Geum-
dc.contributor.authorHwang, Taeyoung-
dc.contributor.authorKim, Hyungsin-
dc.contributor.authorKang, Shin-Hyuk-
dc.contributor.authorDho, Yun-Sik-
dc.contributor.authorPark, Sung-Hye-
dc.contributor.authorKim, Sang Jeong-
dc.contributor.authorPark, Chul-Kee-
dc.date.accessioned2017-11-23T07:27:53Z-
dc.date.available2017-11-23T16:28:49Z-
dc.date.issued2017-11-17-
dc.identifier.citationCancer Cell International, 17(1):104ko_KR
dc.identifier.issn1475-2867-
dc.identifier.urihttps://hdl.handle.net/10371/138399-
dc.description.abstractAbstract

Background
Glioblastoma multiforme (GBM) is known as one of the most fatal forms of cancer. MicroRNAs have been widely implicated in the regulation of mammalian development and pathogenesis. The brain-enriched miR-29 subfamilies are known to be exclusively expressed in the developing brain, and they are aberrantly down-regulated in GBM. This study aims to elucidate the role of miR-29b in GBM development and the feasibility of therapeutic targeting using conjugated nanoparticles.

Methods
After confirmation of miR-29b expression levels in GBM tissues by analysis of open source data, the anticancer effect of miR-29b was tested by the introduction of syn-hsa-miR-29b-3p in the A172 GBM cell line. In vitro studies of cell viability and apoptosis and ex vivo study using GBM tissue slice cultures from 3 patients and nanoparticle delivery of miR-29b were performed.

Results
We discovered an increase in apoptotic cell populations with the introduction of miR-29b in the GBM cell line. An established human-derived GBM tissue slice culture system confirmed the anticancer effect of miR-29b-conjugated nanoparticles. Using PCR array, we found that exogenous miR-29b inhibits the expression of COL1A2, COL3A1, COL4A1, ELN, ITGA11, MMP24, and SPARC, which mediates an anticancer effect.

Conclusions
miR-29b may serve as a putative therapeutic molecule when its expression is restored using a nanoparticle delivery system in GBM.
ko_KR
dc.description.sponsorshipThis study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015R1D1A1A09057171) in Korea and the Seoul National University Hospital Research Fund (03-2015-0160).ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.subjectGlioblastomako_KR
dc.subjectmiR-29bko_KR
dc.subjectAnti-cancer effectko_KR
dc.subjectNanoparticleko_KR
dc.titleRestoration of miR-29b exerts anti-cancer effects on glioblastomako_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor신재경-
dc.contributor.AlternativeAuthor심현금-
dc.contributor.AlternativeAuthor황태영-
dc.contributor.AlternativeAuthor김형신-
dc.contributor.AlternativeAuthor강신혁-
dc.contributor.AlternativeAuthor도윤식-
dc.contributor.AlternativeAuthor박성혜-
dc.contributor.AlternativeAuthor김상정-
dc.contributor.AlternativeAuthor박철기-
dc.identifier.doi10.1186/s12935-017-0476-9-
dc.language.rfc3066en-
dc.rights.holderThe Author(s)-
dc.date.updated2017-11-19T04:28:35Z-
Appears in Collections:
Files in This Item:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share