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Complex Intratissue Microbiota Forms Biofilms in Periodontal Lesions
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Baek, K. | en |
dc.contributor.author | Ji, S. | - |
dc.contributor.author | Choi, Y. | - |
dc.creator | 최영님 | - |
dc.date.accessioned | 2018-01-24T06:02:50Z | - |
dc.date.available | 2018-01-25T10:22:25Z | - |
dc.date.issued | 2017-10 | - |
dc.identifier.citation | Journal of Dental Research, Vol.97 No.2, pp. 192-200 | - |
dc.identifier.issn | 0022-0345 | - |
dc.identifier.uri | https://hdl.handle.net/10371/139296 | - |
dc.description.abstract | Periodontitis is caused by dysbiotic subgingival bacterial communities that may lead to increased bacterial invasion into gingival tissues. Although shifts in community structures associated with transition from health to periodontitis have been well characterized, the nature of bacteria present within the gingival tissue of periodontal lesions is not known. To characterize microbiota within tissues of periodontal lesions and compare them with plaque microbiota, gingival tissues and subgingival plaques were obtained from 7 patients with chronic periodontitis. A sequencing analysis of the 16S rRNA gene revealed that species richness and diversity were not significantly different between the 2 groups. However, intersubject variability of intratissue communities was smaller than that of plaque communities. In addition, when compared with the plaque communities, intratissue communities were characterized by decreased abundance of Firmicutes and increased abundance of Fusobacteria and Chloroflexi. In particular, Fusobacterium nucleatum and Porphyromonas gingivalis were highly enriched within the tissue, composing 15% to 40% of the total bacteria. Furthermore, biofilms, as visualized by alcian blue staining and atomic force microscopy, were observed within the tissue where the degradation of connective tissue fibers was prominent. In conclusion, very complex bacterial communities exist in the form of biofilms within the gingival tissue of periodontal lesions, which potentially serve as a reservoir for persistent infection. This novel finding may prompt new research on therapeutic strategies to treat periodontitis. | - |
dc.description.sponsorship | We acknowledge professor Hyun-Man Kim at the Department of Cell and Developmental Biology for providing histologic expertise.
A supplemental appendix to this article is available online. This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (2016-929358 and 2016R1E1A1A01942402). The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article. | - |
dc.language.iso | en | en |
dc.publisher | SAGE PUBLICATIONS INC | en |
dc.subject | Complex Intratissue Microbiota Forms Biofilms in Periodontal Lesions | en |
dc.subject | 의약학 | en |
dc.subject | periodontitis | - |
dc.subject | gingiva | - |
dc.subject | microbial ecology | - |
dc.subject | bacteria | - |
dc.subject | Fusobacterium nucleatum | - |
dc.title | Complex Intratissue Microbiota Forms Biofilms in Periodontal Lesions | en |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 최영님 | - |
dc.identifier.doi | 10.1177/0022034517732754 | - |
dc.description.srnd | OAIID:RECH_ACHV_DSTSH_NO:T201715478 | - |
dc.description.srnd | RECH_ACHV_FG:RR00200001 | - |
dc.description.srnd | ADJUST_YN: | - |
dc.description.srnd | EMP_ID:A075701 | - |
dc.description.srnd | CITE_RATE:4.755 | - |
dc.description.srnd | FILENAME:JDR.pdf | - |
dc.description.srnd | DEPT_NM:치의과학과 | - |
dc.description.srnd | EMAIL:youngnim@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | FILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/e627bb9f-08eb-4ec0-a76e-80fc87a0cf10/link | - |
dc.description.srnd | CONFIRM:Y | - |
dc.identifier.srnd | T201715478 | - |
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