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Studies on the role of cell division during neuronal differentiation in P19 EC cells
DC Field | Value | Language |
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dc.contributor.advisor | 이건수 | - |
dc.contributor.author | 박조해 | - |
dc.date.accessioned | 2018-05-28T17:09:51Z | - |
dc.date.available | 2018-05-28T17:09:51Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.other | 000000150816 | - |
dc.identifier.uri | https://hdl.handle.net/10371/141125 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 자연과학대학 생명과학부, 2018. 2. 이건수. | - |
dc.description.abstract | Cell cycle progression must be tightly coordinated with cell fate choice. In this regard, cell division is one of the crucial factors that ensure differentiation process, as exemplified by mitotic clonal expansion during adipogenesis. However, there is not much of evidence and underlying mechanism that help us understand how cell division might play a role in other types of differentiation, ensuring controlled tissue development and homeostasis. Here, I focused on the involvement of cell division during neuronal differentiation. I used retinoic acid (RA)-induced in vitro neurogenesis system of P19 embryonic carcinoma cells to examine the direct link between cell division and neuronal differentiation. I observed that cell cycle blockers inhibited neuronal differentiation of P19 cells. In order to investigate the underlying mechanisms, I screened for RA target genes whose transcripts were reduced with cell cycle blockers and identified Tshz1 as a candidate for the cell division-dependent genes. The promoter analysis of Tshz1 found the minimal essential region for RA and cell division-dependent transcriptional activation. Through computational sequence analysis of the promoter, E2F1 was predicted a possible upstream transcription factor for Tshz1. Furthermore, the E2F1 binding activity on the Tshz1 promoter was reduced with the thymidine treatment. Taken together, E2F1 may function as a transcription factor whose activity is controlled in a cell division-specific manner for RA induction of Tshz1 expression. This study is an example that cell division itself functions as a regulatory mechanism to ensure neuronal differentiation. | - |
dc.description.tableofcontents | Background. 1
1. Cell cycle control during differentiation. 2 1.1 Cell cycle structure of pluripotent vs. differentiated cells. 2 1.2 G1 phase during differentiation. 3 2. Cell division during differentiation. 5 2.1 Mitotic clonal expansion (MCE) during adipogenesis 4 2.2 Cell proliferation during myogenesis. 5 2.3 DNA replication and temporal gene-activation. 6 3. Neuronal differentiation of P19 cells 7 3.1 P19 embryonic carcinoma cells. 7 3.2 Retinoic acid-induced transcription. 8 3.3 Studying the role of cell division in P19 cells. 9 Title: Cell division-dependent control during neuronal differentiation in P19 EC cells Abstract. 17 Introduction 20 Materials and Methods 27 Antibodies 27 Cell culture and drug treatment 27 In vitro neuronal differentiation 28 Immunocytochemistry analysis 28 Immunoblot analysis 29 RT-qPCR 30 Luciferase assay 30 Electrophoretic mobility shift assay (EMSA) 31 FACS analysis 32 Chromatin immunoprecipitation assay 32 Neurite outgrowth assay 32 Statistical analysis 34 Results 36 Part 1. Cell division control at an early stage of neuronal differentiation in P19 cells. 36 Importance of cell division at an early stage of differentiation for neuronal differentiation and neuron morphogenesis. 38 Part 2. Identification of RA-induced genes that are regulated in a cell division-dependent manner in P19 cells 40 Identification of Tshz1 promoter region that is important for RA and cell division-dependent activation. 43 Putative transcription factors may bind to the -1250/-1226 sequence of Tshz1 promoter in a RA- and cell division-dependent manner 45 E2F1 may be transcription factor that regulates Tshz1 expression in RA and cell division-dependent manner in P19 cells 47 Discussion 79 References. 88 Abstract in Korean 94 | - |
dc.format | application/pdf | - |
dc.format.extent | 2355090 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Stem cell | - |
dc.subject | Differentiation | - |
dc.subject | Cell division | - |
dc.subject | Retinoic acid | - |
dc.subject | Transcription factor | - |
dc.subject | Neurogenesis | - |
dc.subject | P19 cell | - |
dc.subject.ddc | 570 | - |
dc.title | Studies on the role of cell division during neuronal differentiation in P19 EC cells | - |
dc.type | Thesis | - |
dc.description.degree | Doctor | - |
dc.contributor.affiliation | 자연과학대학 생명과학부 | - |
dc.date.awarded | 2018-02 | - |
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