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Acquisition of pluripotency in the chick embryo occurs during intrauterine embryonic development via a unique transcriptional network

Cited 6 time in Web of Science Cited 6 time in Scopus
Authors
Han, Jae Yong; Lee, Hyo Gun; Park, Young Hyun; Hwang, Young Sun; Kim, Sang Kyung; Rengaraj, Deivendran; Cho, Byung Wook; Lim, Jeong Mook
Issue Date
2018-04-10
Publisher
BioMed Central
Citation
Journal of Animal Science and Biotechnology, 9(1):31
Keywords
AvianEmbryonic developmentNANOGPluripotencyTranscriptional factor
Abstract
Background
Acquisition of pluripotency by transcriptional regulatory factors is an initial developmental event that is required for regulation of cell fate and lineage specification during early embryonic development. The evolutionarily conserved core transcriptional factors regulating the pluripotency network in fishes, amphibians, and mammals have been elucidated. There are also species-specific maternally inherited transcriptional factors and their intricate transcriptional networks important in the acquisition of pluripotency. In avian species, however, the core transcriptional network that governs the acquisition of pluripotency during early embryonic development is not well understood.

Results
We found that chicken NANOG (cNANOG) was expressed in the stages between the pre-ovulatory follicle and oocyte and was continuously detected in Eyal-Giladi and Kochav stage I (EGK.I) to X. However, cPOUV was not expressed during folliculogenesis, but began to be detectable between EGK.V and VI. Unexpectedly, cSOX2 could not be detected during folliculogenesis and intrauterine embryonic development. Instead of cSOX2, cSOX3 was maternally inherited and continuously expressed during chicken intrauterine development. In addition, we found that the pluripotency-related genes such as cENS-1, cKIT, cLIN28A, cMYC, cPRDM14, and cSALL4 began to be dramatically upregulated between EGK.VI and VIII.

Conclusion
These results suggest that chickens have a unique pluripotent circuitry since maternally inherited cNANOG and cSOX3 may play an important role in the initial acquisition of pluripotency. Moreover, the acquisition of pluripotency in chicken embryos occurs at around EGK.VI to VIII.
ISSN
2049-1891
Language
English
URI
https://hdl.handle.net/10371/141237
DOI
https://doi.org/10.1186/s40104-018-0246-0
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Journal Papers (저널논문_농생명공학부)
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