Effect of 3D PEG Scaffold Hybridized with MC3T3-E1 Cell-derived Extracellular Matrix on Osteogenesis of Human Mesenchymal Stem Cell

Abstract

Existing studies about utilizing extracellular matrix (ECM) has focused on organs obtained from living organisms. But there is limitation in supply of organs has limited supply and the composition and therapeutic efficacy of ECMs has distribution depending on the factors represented as donors health status and age. In contrast, the extracellular matrix obtained from the cell line cultured in the laboratory has the advantage

it could be produced under controlled environment with uniform quality. In this study, we developed an optimized decellularization process to separate the ECM of osteoblast cell line, MC3T3-E1. And we cultured human bone marrow-derived mesenchymal stem cells with the hydrolyzed ECM to evaluate bone-differentiation ability. Osteogenesis of the stem cell was analyzed by ALP staining, alizarin red S staining and real-time PCR. As a result, we confirmed the statistically significant osteoinductivity of the ECM produced by MC3T3-E1 cell line in 2D and 3D condition. Our study suggests that ECM derived from cell lines could be utilized in tissue engineering fields as useful biomaterial.