S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Agricultural Biotechnology (농생명공학부) Theses (Master's Degree_농생명공학부)
The Novel Effects of 5-(3',4-Dihydroxyphenyl)-γ- valerolactone, a major Metabolite of Procyanidins, on C2C12 Murine Myoblasts Myogenesis
DHPV의 노화성 근감소 억제 및 근육분화 촉진 효능
- 농업생명과학대학 농생명공학부
- Issue Date
- 서울대학교 대학원
- 학위논문 (석사)-- 서울대학교 대학원 : 농업생명과학대학 농생명공학부, 2018. 2. 이기원.
- Procyanidins have many health benefits. After intake, procyanidins are degraded into small metabolites by gastrointestinal microorganisms. According to a previous study, 5-(3',4-Dihydroxyphenyl)-γ-valerolactone is the most abundant among these metabolites. The object of this study is to determine the effects of DHPV on muscle and whether DHPV could recover aged muscle to normal. TGF-β is connected to muscle aging and attenuates the muscle differentiation and inhibits myogenesis.
In this study, I measured myoD, myogenin, and the myosin heavy chain expression level to determine whether DHPV improved the myogenesis of C2C12, which had been inhibited by a TGF-β treatment. The results show that the RNA and protein expression level of these 3 biomarkers increases in the DHPV treated group in comparison to TGF-β only treated. In addition, DHPV modulated the TGF-β signaling pathway and C2C12 myogenesis by regulating p-smad2/3, p-JNK, p-ERK and p-p38 expression. Taken together, it may be suggested that DHPV is an ideal therapeutic candidate for recovering myogenesis and muscle differentiation evoked by aging.