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Genetic analysis of a Nonpungent EMS mutant in pepper (Capsicum annuum L.) : 무신미 EMS 돌연변이체의 유전적 분석
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 강병철 | - |
dc.contributor.author | 이주헌 | - |
dc.date.accessioned | 2018-05-29T03:59:19Z | - |
dc.date.available | 2018-05-29T03:59:19Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.other | 000000151240 | - |
dc.identifier.uri | https://hdl.handle.net/10371/141817 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 농업생명과학대학 식물생산과학부, 2018. 2. 강병철. | - |
dc.description.abstract | Capsaicinoid is the alkaloid compounds produced in peppers
(Capsicum spp.). They are responsible for pepper pungency or hotness and is one of the important traits in breeding programs. Although many studies have been performed to elucidate its biosynthesis, the biosynthetic pathway is largely based on studies on the similar pathways of other plants. To understand the biosynthesis of capsaicinoid, a nonpungent mutant 221-2-1a, developed from pungent 'Yuwol-cho' were analyzed. 221-2-1a was found to have no mutation in the coding sequence of Pun1, but the levels of capsaicinoid in their fruits were drastically decreased compared to that of Yuwol-cho. To identify the gene(s) responsible for the non-pungent trait in 221-2-1a. Gene expressions of 12 genes involved in capsaicinoid biosynthesis were compared between 221-2-1a and Yuwol-cho together with several selected cultivars. Seven out of 12 genes (pAMT, BCAT, ACL, KAS, FatA, PAL, and Pun1) showed a significant decrease in their expression levels in 221-2-1a compared to pungent cultivars. Furthermore, the inheritance of pungency was studied in a population derived from a between Yuwolcho and 221-2-1a. The inheritance study showed that the nonpungency in 221-2-1a is controlled by two recessive genes. To identify the genes responsible for non-pungency trait, samples of Yuwol-cho and bulked F3 were sequenced and analyzed by MutMap. A total of 11 SNPs were identified in the intergenic sequences and the candidates were annotated. Although candidate genes were not capsaicinoid biosynthesic genes, the candidate genes are believed to be ideal targets in studies to carryout in the future. | - |
dc.description.tableofcontents | I. LITERATURE REVIEW 01
II. INTRODUCTION 07 III. MATERIALS AND METHODS 10 3.1 Plant materials 10 3.2 Sample preparation for capsaicinoid analysis.. 10 3.3 Gibbs screening and HPLC analysis . 11 3.4 Isolation of RNA and cDNA synthesis 13 3.5 Quantitative real-time PCR analysis. 13 3.6 Polymerase chain reaction (PCR) amplification and sequence analysis of Pun1. 17 3.7 Genomic DNA extraction 17 3.8 Whole genome sequencing of wild type and mutant bulk 18 3.9 Alignment of reference sequence and MutMap.. 18 IV. RESULTS 20 4.1 Capsaicinoid measurement in placenta tissues . 20 4.2 Expression analysis of capsaicinoid pathway genes. 23 4.3 Comparison of Pun1 exon sequences . 28 4.4 Evaluation of pungency segregation. 30 4.5 Sequencing of Yuwol-cho and bulked F3 DNA 34 4.6 MutMap analysis for candidate region identification 36 V. DISCUSSION 40 VI. REFERENCES. 44 VII. ABSTRACT IN KOREAN 55 VIII. APPENDIX. 57 | - |
dc.format | application/pdf | - |
dc.format.extent | 2180826 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Capsaicinoid | - |
dc.subject | MutMap | - |
dc.subject | Mutation | - |
dc.subject | EMS | - |
dc.subject | Pungency | - |
dc.subject.ddc | 633 | - |
dc.title | Genetic analysis of a Nonpungent EMS mutant in pepper (Capsicum annuum L.) | - |
dc.title.alternative | 무신미 EMS 돌연변이체의 유전적 분석 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 농업생명과학대학 식물생산과학부 | - |
dc.date.awarded | 2018-02 | - |
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