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The difference on the third generation cephalosporin resistance in Escherichia coli from raw milk : Ceftiofur의 사용에 따른 국내 원유 분리 Escherichia coli의 3세대 세팔로스포린계 항생제에 대한 내성 차이
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 박용호 | - |
dc.contributor.author | 김재희 | - |
dc.date.accessioned | 2018-12-03T01:54:10Z | - |
dc.date.available | 2018-12-03T01:54:10Z | - |
dc.date.issued | 2018-08 | - |
dc.identifier.other | 000000152817 | - |
dc.identifier.uri | https://hdl.handle.net/10371/144181 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 수의과대학 수의학과, 2018. 8. 박용호. | - |
dc.description.abstract | The antimicrobial resistance to the third-generation cephalosporins (3G-Ceph) in gram negative bacteria is an emerging concern worldwide. The prevalence of 3G-Ceph resistant bacteria in livestock has also increased in the past a few years in Korea. It is important to elucidate the relatedness between the use of 3G-Ceph and the increased antimicrobial resistance to these antimicrobials in local farms. The current study was performed to evaluate the effect of 3G-Ceph (ceftiofur) use on the increase of antimicrobial resistance by comparing the antimicrobial resistant rates between 3G-Ceph (ceftiofur) using and non-using farms. A total of 160 Escherichia coli (123 from using and 37 from non-using farms) were isolated from raw milk. The antimicrobial resistant rate to 3G-Ceph was significantly higher in 3G-Ceph using farms (9.8%) than in non-using farms (2.7%). The MICs of Ceftriaxone, Cefotaxime were measured as less than 4 ug/ml in isolates from non-using farms while 64-1024 ug/ml from using farms. All the resistant isolates from using farms harbored a CTX-M gene, whereas none from non-using farms. Contaminated raw milk can be a vehicle for transmission of antimicrobial resistant bacteria from dairy to humans. The current study indicates the overuse of 3G-Ceph in dairy farms could increase the risk of transmission of 3G-Ceph resistant bacteria to a human community. Therefore, prudent use of this category of antimicrobials in dairy farms is warranted to prevent the dissemination of 3G-Ceph bacteria from animals to humans. | - |
dc.description.tableofcontents | Abstract 1
Contents 3 List of figure 5 List of tables 6 Abbreviations 7 I. Introduction 8 II. Materials and methods 11 1. Sampling and bacterial isolation 11 2. Identification and antimicrobial susceptibility tests of 3G-Ceph resistant E. coli 12 3. MICs of 3G-Cephs (Ceftiofur, Ceftriaxone, Cefotaxime) 12 4. Detection of blaCTX-M genes 13 5. Pulsed-Field Gel Electrophoresis (PFGE) 13 III. Results 14 1. Isolation of 3G-Ceph resistant E. coli from raw milk 14 2. Susceptibility of 3G-Ceph-resistant E.coli isolates to other antimicrobials 14 3. Determination of MICs 15 4. Detection of blaCTX-M genes 15 5. Genotyping of 3G-Ceph resistant E. coli by PFGE 15 IV. Discussion 16 References 18 국문초록 29 | - |
dc.format | application/pdf | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject.ddc | 636.089 | - |
dc.title | The difference on the third generation cephalosporin resistance in Escherichia coli from raw milk | - |
dc.title.alternative | Ceftiofur의 사용에 따른 국내 원유 분리 Escherichia coli의 3세대 세팔로스포린계 항생제에 대한 내성 차이 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Jae Hee Kim | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 수의과대학 수의학과 | - |
dc.date.awarded | 2018-08 | - |
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