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Generation, culture, and differentiation of human embryonic stem cells for therapeutic applications

Cited 37 time in Web of Science Cited 40 time in Scopus
Authors
Moon, Shin Yong; Park, Yong Bin; Kim, Dae-Sung; Oh, Sun Kyung; Kim, Dong-Wook
Issue Date
2005-10-26
Publisher
Elsevier
Citation
Mol Ther. 2006 Jan;13(1):5-14. Epub 2005 Oct 20.
Keywords
Cell LineageCells, CulturedAnimalsBlastocyst/*cytology*Cell DifferentiationGene TherapyGerm Layers/*cytologyHumansMiceStem Cell TransplantationStem Cells/*cytology
Abstract
Embryonic stem (ES) cells, derived from the inner cell mass of the mammalian blastocyst, can continuously proliferate in an undifferentiated state and can also be induced to differentiate into a desired cell lineage. These abilities make ES cells an appealing source for cell replacement therapies, the study of developmental biology, and drug/toxin screening studies. As compared to mouse ES cells, human ES cells have only recently been derived and studied. Although there are many differences in properties between mouse and human ES cells, the study of mouse ES cells has provided important insights into human ES cell research. In this review, we describe the advantages and disadvantages of methods used for human ES cell derivation, the expansion of human ES cells, and the current status of human ES cell differentiation research. In addition, we discuss the endeavor that scientists have undertaken toward the therapeutic application of these cells, which includes therapeutic cloning and the improvement of human ES cell culture conditions.
ISSN
1525-0016 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16242999

http://hdl.handle.net/10371/14768
DOI
https://doi.org/10.1016/j.ymthe.2005.09.008
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Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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