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Reactive blue 2, an antagonist of rat P2Y4, increases K+ secretion in rat cochlea strial marginal cells
Cited 2 time in
Web of Science
Cited 3 time in Scopus
- Authors
- Issue Date
- 2006-07-15
- Publisher
- Elsevier
- Citation
- Hear Res. 2006 Sep;219(1-2):66-73. Epub 2006 Jul 12.
- Keywords
- 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacology ; Adenosine Triphosphate/analogs & derivatives/pharmacology ; Animals ; Dose-Response Relationship, Drug ; Electrophysiology ; Enzyme Inhibitors/*pharmacology ; Ion Transport/drug effects/physiology ; Perilymph/metabolism ; Potassium/*metabolism ; Rats ; Receptors, Purinergic P2/agonists/*antagonists & inhibitors ; Sodium-Potassium-Exchanging ATPase/metabolism ; Stria Vascularis/cytology/*drug effects/secretion ; Suramin/pharmacology ; Triazines/*pharmacology ; Uridine Triphosphate/pharmacology ; Vibration
- Abstract
- Extracellular ATP decreases K+ secretion in strial marginal cells via apical P2Y4 receptors. We investigated the effect of reactive blue 2 (RB-2), an antagonist of rat P2Y4, on rat strial marginal cells using a voltage-sensitive vibrating probe. The application of RB-2 increased K+ secretion in a dose-dependent manner, and this increase was characterized as a peak followed by a partial relaxation to a steady-state. Moreover, this response was similar to that caused by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). Suramin had no similar effect, except at high concentration. Thus, we tested the effects of these chemicals on P2Y4 receptors in strial marginal cells. Both RB-2 and DIDS had antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion. Interestingly, 2'- and 3'-O-(4-benzoyl-benzoyl)adenosine 5'-triphosphate (BzATP) exhibited an agonistic effect at P2Y4 receptor, which was blocked by RB-2, but not by pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS). Based on these results, we speculate that direct and/or indirect inhibitory mechanisms between P2Y4 and KENQ1/KCNE1 K+ channels exist in strial marginal cell.
- ISSN
- 0378-5955 (Print)
- Language
- English
- URI
- http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T73-4KCXJF1-6&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=cf4ad093796809579b070a3b08a29290
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16839719
https://hdl.handle.net/10371/14775
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