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Difference in suitable mechanical properties of three-dimensional, synthetic scaffolds for self-renewing mouse embryonic stem cells of different genetic backgrounds
DC Field | Value | Language |
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dc.contributor.author | Lee, Myongook | - |
dc.contributor.author | Ahn, Jong Il | - |
dc.contributor.author | Ahn, Ji Yeon | - |
dc.contributor.author | Yang, Woo Sub | - |
dc.contributor.author | Hubbell, Jeffrey A. | - |
dc.contributor.author | Lim, Jeong Mook | - |
dc.contributor.author | Lee, Seung Tae | - |
dc.creator | 임정묵 | - |
dc.date.accessioned | 2019-04-25T01:49:21Z | - |
dc.date.available | 2020-04-05T01:49:21Z | - |
dc.date.created | 2018-04-12 | - |
dc.date.created | 2018-04-12 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.citation | Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol.105 No.8, pp.2261-2268 | - |
dc.identifier.issn | 1552-4973 | - |
dc.identifier.uri | https://hdl.handle.net/10371/149661 | - |
dc.description.abstract | We evaluated whether the genetic background of embryonic stem cells (ESCs) affects the properties suitable for three-dimensional (3D) synthetic scaffolds for cell self-renewal. Inbred R1 and hybrid B6D2F1 mouse ESC lines were cultured for 7 days in hydrogel scaffolds with different properties derived from conjugating 7.5, 10, 12.5, or 15% (wt/vol) vinylsulfone-functionalized three-, four-, or eight-arm polyethylene glycol (PEG) with dicysteine-containing crosslinkers with an intervening matrix metalloproteinase-specific cleavage sites. Cell proliferation and expression of self-renewal-related genes and proteins by ESCs cultured in feeder-free or containing 2D culture plate or 3D hydrogel were monitored. As a preliminary experiment, the E14 ESC-customized synthetic 3D microenvironment did not maintain self-renewal of either the R1 or B6D2F1 ESCs. The best R1 cell proliferation (10.04 vs. 0.16-4.39; p<0.0001) was observed in the four-arm 7.5% PEG-based hydrogels than those with other properties, whereas the F1 ESCs showed better proliferation when they were embedded in the three-arm 10% hydrogels. Self-renewal-related gene and protein expression by ESCs after feeder-free 3D culture was generally maintained compared with the feeder-containing 2D culture, but expression patterns and quantities differed. However, the feeder-free 3D culture yielded better expression than the feeder-free 2D culture. In conclusion, genetic background determined the suitability of hydrogel scaffolds for self-renewal of ESCs, which requires customization for the mechanical properties of each cell line. (C) 2016 Wiley Periodicals, Inc. | - |
dc.language | 영어 | - |
dc.language.iso | en | en |
dc.publisher | John Wiley & Sons Inc. | - |
dc.title | Difference in suitable mechanical properties of three-dimensional, synthetic scaffolds for self-renewing mouse embryonic stem cells of different genetic backgrounds | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/jbm.b.33763 | - |
dc.citation.journaltitle | Journal of Biomedical Materials Research - Part B Applied Biomaterials | - |
dc.identifier.wosid | 000412194200007 | - |
dc.identifier.scopusid | 2-s2.0-84979656452 | - |
dc.description.srnd | OAIID:RECH_ACHV_DSTSH_NO:T201612791 | - |
dc.description.srnd | RECH_ACHV_FG:RR00200001 | - |
dc.description.srnd | ADJUST_YN: | - |
dc.description.srnd | EMP_ID:A074417 | - |
dc.description.srnd | CITE_RATE:3.373 | - |
dc.description.srnd | FILENAME:Lee_et_al-2016-Journal_of_Biomedical_Materials_Research_Part_B-_Applied_Biomaterials (1).pdf | - |
dc.description.srnd | DEPT_NM:농생명공학부 | - |
dc.description.srnd | EMAIL:limjm@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | FILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/a4d91545-7e50-4ff9-a8f4-715a0c8b9d6f/link | - |
dc.citation.endpage | 2268 | - |
dc.citation.number | 8 | - |
dc.citation.startpage | 2261 | - |
dc.citation.volume | 105 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Lim, Jeong Mook | - |
dc.identifier.srnd | T201612791 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | ESTABLISHMENT | - |
dc.subject.keywordPlus | GENOTYPE | - |
dc.subject.keywordAuthor | embryonic stem cells | - |
dc.subject.keywordAuthor | three-dimensional synthetic hydrogel scaffold | - |
dc.subject.keywordAuthor | polyethylene glycol-based hydrogel | - |
dc.subject.keywordAuthor | genetic background | - |
dc.subject.keywordAuthor | self-renewal | - |
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