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Microarray gene expression profiling for predicting complete response to preoperative chemoradiotherapy in patients with advanced rectal cancer

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dc.contributor.authorKim, Il-Jin-
dc.contributor.authorLim, Seok-Byung-
dc.contributor.authorKang, Hio Chung-
dc.contributor.authorChang, Hee Jin-
dc.contributor.authorAhn, Sun-A-
dc.contributor.authorPark, Hye-Won-
dc.contributor.authorJang, Sang-Geun-
dc.contributor.authorPark, Jae-Hyun-
dc.contributor.authorKim, Dae Yong-
dc.contributor.authorJung, Kyung Hae-
dc.contributor.authorChoi, Hyo Seong-
dc.contributor.authorJeong, Seung-Yong-
dc.contributor.authorSohn, Dae Kyung-
dc.contributor.authorKim, Duck-Woo-
dc.contributor.authorPark, Jae-Gahb-
dc.date.accessioned2009-11-25T05:11:20Z-
dc.date.available2009-11-25T05:11:20Z-
dc.date.issued2007-08-01-
dc.identifier.citationDis Colon Rectum. 2007 Sep;50(9):1342-53en
dc.identifier.issn0012-3706 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17665260-
dc.identifier.urihttp://hdl.handle.net/10371/15272-
dc.description.abstractPURPOSE: Preoperative chemoradiotherapy is widely used to improve local control and sphincter preservation in patients with locally advanced rectal cancer. In the present study, we investigated whether microarray gene expression analysis could predict complete response to preoperative chemoradiotherapy in rectal cancer. METHODS: Tumor tissues were obtained from 46 patients with rectal cancer (31 for training and 15 for validation testing). All patients underwent preoperative chemoradiotherapy involving 50.4 gray radiotherapy, followed by surgical excision 6 weeks later. Response to chemoradiotherapy was evaluated according to Dworak's tumor regression grade. Tumor regression Grades 1, 2, and 3 were considered partial responses, and tumor regression Grade 4 was considered a complete response. By using the 31 training samples, genes differentially expressed between partial response and complete response were identified, and clustering analysis was performed. Prediction analysis of response to chemoradiotherapy was performed on the 31 training samples by using a selected set of 95 "predictor" genes. Those findings were validated by independent analysis of the 15 test samples. RESULTS: The 31 training samples comprised 20 partial response and 11 complete response cases. A primary set of 261 genes was identified as differentiating between partial response and complete response. By supervised clustering using these 261 genes, 30 of 31 training samples were clustered correctly according to tumor response. A gene set comprising the top-ranked 95 genes displaying differential expression between partial response and complete response was applied to predict response to chemoradiotherapy. Complete response and partial response were accurately predicted in 84 percent (26/31) of training samples and 87 percent (13/15) of validation samples. CONCLUSIONS: Microarray gene expression analysis was successfully used to predict complete responses to preoperative chemoradiotherapy in patients with advanced rectal cancer.en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.subjectAdulten
dc.subjectAgeden
dc.subjectColectomyen
dc.subjectDNA, Neoplasm/*geneticsen
dc.subjectFemaleen
dc.subjectFollow-Up Studiesen
dc.subjectGene Expression Profilingen
dc.subject*Gene Expression Regulation, Neoplasticen
dc.subjectHumansen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectOligonucleotide Array Sequence Analysis/*methodsen
dc.subjectPredictive Value of Testsen
dc.subjectRectal Neoplasms/drug therapy/radiotherapy/surgery/*therapyen
dc.subjectReproducibility of Resultsen
dc.subjectTreatment Outcomeen
dc.titleMicroarray gene expression profiling for predicting complete response to preoperative chemoradiotherapy in patients with advanced rectal canceren
dc.typeArticleen
dc.contributor.AlternativeAuthor김일진-
dc.contributor.AlternativeAuthor임석병-
dc.contributor.AlternativeAuthor강효정-
dc.contributor.AlternativeAuthor장희진-
dc.contributor.AlternativeAuthor안선아-
dc.contributor.AlternativeAuthor박혜원-
dc.contributor.AlternativeAuthor장상근-
dc.contributor.AlternativeAuthor박재현-
dc.contributor.AlternativeAuthor김대용-
dc.contributor.AlternativeAuthor정경해-
dc.contributor.AlternativeAuthor최효성-
dc.contributor.AlternativeAuthor정승용-
dc.contributor.AlternativeAuthor손대경-
dc.contributor.AlternativeAuthor김덕우-
dc.contributor.AlternativeAuthor박재갑-
dc.identifier.doi10.1007/s10350-007-277-7-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
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