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BetaPix-a enhances the activity of phospholipase Cgamma1 by binding SH3 domain in breast cancer

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dc.contributor.authorBae, Ji-Yeon-
dc.contributor.authorAhn, Soo-Jung-
dc.contributor.authorLee, Jeong Eon-
dc.contributor.authorKim, Jung-Eun-
dc.contributor.authorHan, Mi-Ryung-
dc.contributor.authorHan, Wonshik-
dc.contributor.authorKim, Seok Won-
dc.contributor.authorShin, Hyuk Jai-
dc.contributor.authorLee, Seung Joon-
dc.contributor.authorPark, Dongeun-
dc.contributor.authorNoh, Dong-Young-
dc.date.accessioned2009-11-26T04:34:43Z-
dc.date.available2009-11-26T04:34:43Z-
dc.date.issued2004-12-15-
dc.identifier.citationJ Cell Biochem. 2005 Apr 1;94(5):1010-6en
dc.identifier.issn0730-2312 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15597340-
dc.identifier.urihttps://hdl.handle.net/10371/15821-
dc.description.abstractPhospholipase C-gamma1 (PLCgamma1) plays a critical role in cell growth and proliferation by generating the second messengers, diacylglycerol and 1, 4, 5-inositol triphosphate. To investigate the roles of Src homology domain 2 and domain 3 of PLCgamma1 in PLCgamma1-mediated cell signaling, we characterized some proteins binding to these domains in the MCF7 and MDA-MB-231 breast cancer cell lines. Of the several proteins that bind to glutathione-S-transferase-SH2/SH2/SH3, we identified an 85 kDa protein that binds to the SH3 domain of PLCgamma1 as the guanine nucleotide exchange factor, p21-activated protein kinase-interacting exchange factor-a (betaPix-a). BetaPix-a co-immunoprecipitated with PLCgamma1 in breast cancer tissues extracts and in MCF7 and MDA-MB-231 cell extracts. In addition, PDGF-stimulated PLCgamma1 activity was elevated in betaPix-a-overexpressing NIH3T3 cells. Our results suggest that betaPix-a binds to the Src homology domain 3 of PLCgamma1 and promotes tumor growth in breast cancer by enhancing the activity PLCgamma1.en
dc.language.isoen-
dc.publisherJohn Wiley & Sonsen
dc.subjectAnimalsen
dc.subjectBlotting, Westernen
dc.subjectBreast Neoplasms/*enzymology/pathologyen
dc.subjectCell Cycle Proteins/*physiologyen
dc.subjectFemaleen
dc.subjectGuanine Nucleotide Exchange Factors/*physiologyen
dc.subjectHumansen
dc.subjectHydrolysisen
dc.subjectImmunohistochemistryen
dc.subjectMiceen
dc.subjectNIH 3T3 Cellsen
dc.subjectPhospholipase C gammaen
dc.subjectProtein Bindingen
dc.subjectType C Phospholipases/*metabolismen
dc.subjectsrc Homology Domainsen
dc.titleBetaPix-a enhances the activity of phospholipase Cgamma1 by binding SH3 domain in breast canceren
dc.typeArticleen
dc.contributor.AlternativeAuthor배지연-
dc.contributor.AlternativeAuthor안수정-
dc.contributor.AlternativeAuthor이정언-
dc.contributor.AlternativeAuthor김정은-
dc.contributor.AlternativeAuthor한미령-
dc.contributor.AlternativeAuthor한원식-
dc.contributor.AlternativeAuthor김석원-
dc.contributor.AlternativeAuthor신혁재-
dc.contributor.AlternativeAuthor이성준-
dc.contributor.AlternativeAuthor박동은-
dc.contributor.AlternativeAuthor노동영-
dc.identifier.doi10.1002/jcb.20357-
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