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BetaPix-a enhances the activity of phospholipase Cgamma1 by binding SH3 domain in breast cancer
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bae, Ji-Yeon | - |
dc.contributor.author | Ahn, Soo-Jung | - |
dc.contributor.author | Lee, Jeong Eon | - |
dc.contributor.author | Kim, Jung-Eun | - |
dc.contributor.author | Han, Mi-Ryung | - |
dc.contributor.author | Han, Wonshik | - |
dc.contributor.author | Kim, Seok Won | - |
dc.contributor.author | Shin, Hyuk Jai | - |
dc.contributor.author | Lee, Seung Joon | - |
dc.contributor.author | Park, Dongeun | - |
dc.contributor.author | Noh, Dong-Young | - |
dc.date.accessioned | 2009-11-26T04:34:43Z | - |
dc.date.available | 2009-11-26T04:34:43Z | - |
dc.date.issued | 2004-12-15 | - |
dc.identifier.citation | J Cell Biochem. 2005 Apr 1;94(5):1010-6 | en |
dc.identifier.issn | 0730-2312 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15597340 | - |
dc.identifier.uri | https://hdl.handle.net/10371/15821 | - |
dc.description.abstract | Phospholipase C-gamma1 (PLCgamma1) plays a critical role in cell growth and proliferation by generating the second messengers, diacylglycerol and 1, 4, 5-inositol triphosphate. To investigate the roles of Src homology domain 2 and domain 3 of PLCgamma1 in PLCgamma1-mediated cell signaling, we characterized some proteins binding to these domains in the MCF7 and MDA-MB-231 breast cancer cell lines. Of the several proteins that bind to glutathione-S-transferase-SH2/SH2/SH3, we identified an 85 kDa protein that binds to the SH3 domain of PLCgamma1 as the guanine nucleotide exchange factor, p21-activated protein kinase-interacting exchange factor-a (betaPix-a). BetaPix-a co-immunoprecipitated with PLCgamma1 in breast cancer tissues extracts and in MCF7 and MDA-MB-231 cell extracts. In addition, PDGF-stimulated PLCgamma1 activity was elevated in betaPix-a-overexpressing NIH3T3 cells. Our results suggest that betaPix-a binds to the Src homology domain 3 of PLCgamma1 and promotes tumor growth in breast cancer by enhancing the activity PLCgamma1. | en |
dc.language.iso | en | - |
dc.publisher | John Wiley & Sons | en |
dc.subject | Animals | en |
dc.subject | Blotting, Western | en |
dc.subject | Breast Neoplasms/*enzymology/pathology | en |
dc.subject | Cell Cycle Proteins/*physiology | en |
dc.subject | Female | en |
dc.subject | Guanine Nucleotide Exchange Factors/*physiology | en |
dc.subject | Humans | en |
dc.subject | Hydrolysis | en |
dc.subject | Immunohistochemistry | en |
dc.subject | Mice | en |
dc.subject | NIH 3T3 Cells | en |
dc.subject | Phospholipase C gamma | en |
dc.subject | Protein Binding | en |
dc.subject | Type C Phospholipases/*metabolism | en |
dc.subject | src Homology Domains | en |
dc.title | BetaPix-a enhances the activity of phospholipase Cgamma1 by binding SH3 domain in breast cancer | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 배지연 | - |
dc.contributor.AlternativeAuthor | 안수정 | - |
dc.contributor.AlternativeAuthor | 이정언 | - |
dc.contributor.AlternativeAuthor | 김정은 | - |
dc.contributor.AlternativeAuthor | 한미령 | - |
dc.contributor.AlternativeAuthor | 한원식 | - |
dc.contributor.AlternativeAuthor | 김석원 | - |
dc.contributor.AlternativeAuthor | 신혁재 | - |
dc.contributor.AlternativeAuthor | 이성준 | - |
dc.contributor.AlternativeAuthor | 박동은 | - |
dc.contributor.AlternativeAuthor | 노동영 | - |
dc.identifier.doi | 10.1002/jcb.20357 | - |
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