S-Space College of Medicine/School of Medicine (의과대학/대학원) Surgery (외과학전공) Journal Papers (저널논문_외과학전공)
A streamlined platform for high-content functional proteomics of primary human specimens
- Jessani, Nadim; Niessen, Sherry; Wei, BinQing Q; Nicolau, Monica; Humphrey, Mark; Ji, Youngran; Han, Wonshik; Noh, Dong-Young; Yates, John R 3rd; Jeffrey, Stefanie S; Cravatt, Benjamin F
- Issue Date
- Nature Publishing Group
- Nat Methods. 2005 Sep;2(9):691-7
- Breast Neoplasms/diagnosis/*enzymology; Equipment Design; Equipment Failure Analysis; Female; Gene Expression Profiling/*methods; Humans; Neoplasm Proteins/analysis/*metabolism; Proteome/analysis/*metabolism; Proteomics/*instrumentation/methods; Tumor Cells, Cultured; Tumor Markers, Biological/analysis/*metabolism
- Achieving information content of satisfactory breadth and depth remains a formidable challenge for proteomics. This problem is particularly relevant to the study of primary human specimens, such as tumor biopsies, which are heterogeneous and of finite quantity. Here we present a functional proteomics strategy that unites the activity-based protein profiling and multidimensional protein identification technologies (ABPP-MudPIT) for the streamlined analysis of human samples. This convergent platform involves a rapid initial phase, in which enzyme activity signatures are generated for functional classification of samples, followed by in-depth analysis of representative members from each class. Using this two-tiered approach, we identified more than 50 enzyme activities in human breast tumors, nearly a third of which represent previously uncharacterized proteins. Comparison with cDNA microarrays revealed enzymes whose activity, but not mRNA expression, depicted tumor class, underscoring the power of ABPP-MudPIT for the discovery of new markers of human disease that may evade detection by other molecular profiling methods.
- 1548-7091 (Print)
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