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Overexpressing drought-responsive miRNA 156 in rice (Oryza sativa L.) enhances drought stress resistance : 마이크로RNA 156 과발현 벼의 내건성 향상에 대한 연구

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Authors
배주연
Advisor
김주곤
Issue Date
2019-08
Publisher
서울대학교 대학원
Keywords
DroughtOsmiR156OsSPLCRISPR/Cas9narrow-leaf
Description
학위논문(석사)--서울대학교 대학원 :국제농업기술대학원 국제농업기술학과,2019. 8. 김주곤.
Abstract
Globally the earth is facing an extreme climate changes including flood and
high temperatures, changing the earths surface and weather rapidly. These rapid
changes cause a remarkable reduction in plant growth and productivity. The
discovery of micro RNA in plant has open a new field of study in plant
biotechnology. MicroRNAs play a vital role in abiotic stress and crop
improvement by regulating target gene expression. MicroRNA156 (miR156) is a
highly conserved and emerging tool for improving various plant traits including
crop productivity and drought stress tolerance. The aim of this study was to
investigate how miR156 genes interacts with target genes and induce drought
stress tolerance. To analyze its role, we constructed overexpressing miR156 lines
and knockout lines by using CRISPR/Cas9 system. The sequencing analysis,
however, showed no mutation in mature microRNA sequence in both miR156d
and miR156g Cas9-mediated mutation lines. As a result, we proceeded research
with only overexpressing miR156d and miR156g lines. We observed
overexpressing miR156 plants had more tolerant to drought stress than nontransgenic with the higher survival rate and Fv/Fm value. Next, we predicted
some targets and screened genes showing antagonic expression pattern to
miR156d and miR156g by qRT-PCR. Our highest-ranking candidates include
SPL2, SPL17, SPL18, 01t0187200-01 and 09t0513100-01. Based on these results,
it can be concluded that overexpressing miRNA156d and miR156g genes
improve rice drought tolerance by interacting with its target genes. Finally, it is
important to study the interaction between miRNA156 and SPL during flower
induction and seed development and needed to address their role in cell number
and size during early growth stages. This study provides a strong background for
plant biotechnology and crop improvements and offer an important source for
further functional analysis and molecular breeding for drought stress tolerance in
rice.
Language
eng
URI
https://hdl.handle.net/10371/161114

http://dcollection.snu.ac.kr/common/orgView/000000157590
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Graduate School of International Agricultural Technology (국제농업기술대학원)Dept. of International Agricultural Technology (국제농업기술학과)Theses (Master's Degree_국제농업기술학과)
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