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An isoform of the plastid RNA polymerase-associated protein FSD3 negatively regulates chloroplast development

DC Field Value Language
dc.contributor.authorLee, Sangyool-
dc.contributor.authorJoung, Young Hee-
dc.contributor.authorKim, Ju-Kon-
dc.contributor.authorChoi, Yang Do-
dc.contributor.authorJang, Geupil-
dc.date.accessioned2020-03-23T06:19:51Z-
dc.date.available2020-03-23T15:45:59Z-
dc.date.issued2019-11-27-
dc.identifier.citationBMC Plant Biology, 19(1):524ko_KR
dc.identifier.issn1471-2229-
dc.identifier.uri10.1186/s12870-019-2128-9-
dc.identifier.urihttps://hdl.handle.net/10371/164760-
dc.description.abstractBackground
Plastid-encoded RNA polymerase (PEP) plays an essential role in chloroplast development by governing the expression of genes involved in photosynthesis. At least 12 PEP-associated proteins (PAPs), including FSD3/PAP4, regulate PEP activity and chloroplast development by modulating formation of the PEP complex.

Results
In this study, we identified FSD3S, a splicing variant of FSD3; the FSD3 and FSD3S transcripts encode proteins with identical N-termini, but different C-termini. Characterization of FSD3 and FSD3S proteins showed that the C-terminal region of FSD3S contains a transmembrane domain, which promotes FSD3S localization to the chloroplast membrane but not to nucleoids, in contrast to FSD3, which localizes to the chloroplast nucleoid. We also found that overexpression of FSD3S negatively affects photosynthetic activity and chloroplast development by reducing expression of genes involved in photosynthesis. In addition, FSD3S failed to complement the chloroplast developmental defects in the fsd3 mutant.

Conclusion
These results suggest FSD3 and FSD3S, with their distinct localization patterns, have different functions in chloroplast development, and FSD3S negatively regulates expression of PEP-dependent chloroplast genes, and development of chloroplasts.
ko_KR
dc.description.sponsorshipThis work was carried out with the support of the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01323901 and PJ01364301) Rural Development Administration, Republic of Korea, and the National Research Foundation of Korea Grant funded by the Korean Government (MOE) [NRF-2019R1A2C1007103].ko_KR
dc.language.isoenko_KR
dc.publisherBMCko_KR
dc.subjectChloroplast-
dc.subjectPlastid-encoded RNA polymerase-
dc.subjectPEP-associated protein-
dc.subjectFSD3, FSD3S-
dc.subjectAlternative splicing-
dc.titleAn isoform of the plastid RNA polymerase-associated protein FSD3 negatively regulates chloroplast developmentko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor이상율-
dc.contributor.AlternativeAuthor정영희-
dc.contributor.AlternativeAuthor김주곤-
dc.contributor.AlternativeAuthor최양도-
dc.contributor.AlternativeAuthor장규필-
dc.author.alternativenull-
dc.citation.journaltitleBMC Plant Biologyko_KR
dc.language.rfc3066en-
dc.rights.holderThe Author(s).-
dc.date.updated2019-12-01T04:30:22Z-
dc.citation.number1ko_KR
dc.citation.startpage524ko_KR
dc.citation.volume19ko_KR
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Journal Papers (저널논문_농생명공학부)
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