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Human zinc fingers as building blocks in the construction of artificial transcription factors

Cited 142 time in Web of Science Cited 151 time in Scopus
Authors
Bae, Kwang-Hee; Kwon, Young Do; Shin, Hyun-Chul; Hwang, Moon-Sun; Ryu, Eun-Hyun; Park, Kyung-Soon; Yang, Hyo-Young; Lee, Dong-ki; Lee, Yangsoon; Park, Jinwoo; Kwon, Heung Sun; Kim, Hyun-Won; Yeh, Byung-Il; Lee, Hyean-Woo; Sohn, Soon Hyung; Yoon, Joonho; Seol, Wongi; Kim, Jin-Soo
Issue Date
2003-03
Citation
Nature Biotechnology, Vol.21 No.3, pp.275-280
Abstract
We describe methods for generating artificial transcription factors capable of up- or downregulating the expression of genes whose promoter regions contain the target DNA sequences. To accomplish this, we screened zinc fingers derived from sequences in the human genome and isolated 56 zinc fingers with diverse DNA-binding specificities. We used these zinc fingers as modular building blocks in the construction of novel, sequence-specific DNA-binding proteins. Fusion of these zinc-finger proteins with either a transcriptional activation or repression domain yielded potent transcriptional activators or repressors, respectively. These results show that the human genome encodes zinc fingers with diverse DNA-binding specificities and that these domains can be used to design sequence-specific DNA-binding proteins and artificial transcription factors.
ISSN
1087-0156
URI
https://hdl.handle.net/10371/165599
DOI
https://doi.org/10.1038/nbt796
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