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Phenotypic alteration and target gene identification using combinatorial libraries of zinc finger proteins in prokaryotic cells

Cited 38 time in Web of Science Cited 46 time in Scopus
Issue Date
2005-08
Citation
Journal of Bacteriology, Vol.187 No.15, pp.5496-5499
Abstract
We have developed a method with prokaryotic organisms that uses randomized libraries of zinc finger-containing artificial transcription factors to induce phenotypic variations and to identify genes involved in the generation of a specific phenotype of interest. Combining chromatin immunoprecipitation experiments and in silico prediction of target DNA binding sequences for the artificial transcription factors, we identified ubiX, whose down-regulation correlates with the thermotolerance phenotype in Escherichia coli. Our results show that randomized libraries of artificial transcription factors are powerful tools for functional genomic studies.
ISSN
0021-9193
URI
https://hdl.handle.net/10371/165604
DOI
https://doi.org/10.1128/JB.187.15.5496-5499.2005
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