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Enhancing the solubility of recombinant Akt1 in Escherichia coli with an artificial transcription factor library
Cited 4 time in
Web of Science
Cited 4 time in Scopus
- Authors
- Issue Date
- 2006-02
- Publisher
- 한국미생물·생명공학회
- Citation
- Journal of Microbiology and Biotechnology, Vol.16 No.2, pp.299-302
- Abstract
- A combinatorial library of artificial transcription factors (ATFs) was introduced into the bacterial cells that expressed the Akt1-GFP fusion protein. By measuring the level of fluorescence generated by the transformed E coli cells, we were able to obtain clones in which ATFs increased the solubility of the Akt1. Our results show that ATF library is a useful toot for increasing the solubility of selected recombinant proteins in E coli.
- ISSN
- 1017-7825
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