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Enhancing the solubility of recombinant Akt1 in Escherichia coli with an artificial transcription factor library

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Issue Date
2006-02
Citation
Journal of Microbiology and Biotechnology, Vol.16 No.2, pp.299-302
Keywords
recombinant proteinssolubilityartificial transcription factors
Abstract
A combinatorial library of artificial transcription factors (ATFs) was introduced into the bacterial cells that expressed the Akt1-GFP fusion protein. By measuring the level of fluorescence generated by the transformed E coli cells, we were able to obtain clones in which ATFs increased the solubility of the Akt1. Our results show that ATF library is a useful toot for increasing the solubility of selected recombinant proteins in E coli.
ISSN
1017-7825
URI
https://hdl.handle.net/10371/165608
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