Publications

Detailed Information

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

Cited 1426 time in Web of Science Cited 1541 time in Scopus
Authors

Cho, Seung Woo; Kim, Sojung; Kim, Jong Min; Kim, Jin-Soo

Issue Date
2013-03
Publisher
Nature Publishing Group
Citation
Nature Biotechnology, Vol.31 No.3, pp.230-232
Abstract
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
ISSN
1087-0156
URI
https://hdl.handle.net/10371/165629
DOI
https://doi.org/10.1038/nbt.2507
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Related Researcher

  • College of Natural Sciences
  • Department of Chemistry
Research Area Biology and Biochemistry

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share