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Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
Cited 1426 time in
Web of Science
Cited 1541 time in Scopus
- Authors
- Issue Date
- 2013-03
- Publisher
- Nature Publishing Group
- Citation
- Nature Biotechnology, Vol.31 No.3, pp.230-232
- Abstract
- We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
- ISSN
- 1087-0156
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