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Enrichment of cells with TALEN-induced mutations using surrogate reporters

Cited 21 time in Web of Science Cited 21 time in Scopus
Authors

Kim, Young-Hoon; Ramakrishna, Suresh; Kim, Hyongbum; Kim, Jin-Soo

Issue Date
2014-08
Publisher
Academic Press
Citation
Methods, Vol.69 No.1, pp.108-117
Abstract
Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. (C) 2014 Elsevier Inc. All rights reserved.
ISSN
1046-2023
URI
https://hdl.handle.net/10371/165634
DOI
https://doi.org/10.1016/j.ymeth.2014.04.012
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  • College of Natural Sciences
  • Department of Chemistry
Research Area Biology and Biochemistry

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