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Highly efficient RNA-guided base editing in mouse embryos

Cited 193 time in Web of Science Cited 204 time in Scopus
Authors
Kim, Kyoungmi; Ryu, Seuk-Min; Kim, Sang-Tae; Baek, Gayoung; Kim, Daesik; Lim, Kayeong; Chung, Eugene; Kim, Sunghyun; Kim, Jin-Soo
Issue Date
2017-05
Citation
Nature Biotechnology, Vol.35 No.5, pp.435-437
Abstract
Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.
ISSN
1087-0156
URI
https://hdl.handle.net/10371/165681
DOI
https://doi.org/10.1038/nbt.3816
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