Browse

Identification of essential genes in Streptococcus pneumoniae by allelic replacement mutagenesis

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Song, Jae-Hoon; Ko, Kwan Soo; Lee, Ji-Young; Baek, Jin Yang; Oh, Won Sup; Yoon, Ha Sik; Jeong, Jin-Yong; Chun, Jongsik
Issue Date
2005-06
Citation
Molecules and Cells, Vol.19 No.3, pp.365-374
Keywords
allelic replacement mutagenesisessential genesgenomicsnew antimicrobial agentpneumococci
Abstract
To find potential targets of novel antimicrobial agents, we identified essential genes of Streptococcus pneumoniae using comparative genomics and allelic replacement mutagenesis. We compared the genome of S. pneumoniae R6 with those of Bacillus subtilis, Enterococcus faecalis, Escherichia coli, and Staphylococcus aureus, and selected 693 candidate target genes with > 40% amino acid sequence identity to the corresponding genes in at least two of the other species. The 693 genes were disrupted and 133 were found to be essential for growth. Of these, 32 encoded proteins of unknown function, and we were able to identify orthologues of 22 of these genes by genomic comparisons. The experimental method used in this study is easy to perform, rapid and efficient for identifying essential genes of bacterial pathogens.
ISSN
1016-8478
URI
http://hdl.handle.net/10371/166071
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse