Ca2+-Dependent Intracellular Drug Delivery System Developed with "Raspberry-Type" Particles-on-a-Particle Comprising Mesoporous Silica Core and alpha-Synuclein-Coated Gold Nanoparticles

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Lee, Daekyun; Hong, Je Won; Park, Chanyoung; Lee, Hagyeol; Lee, Ji Eun; Hyeon, Taeghwan; Paik, Seung R.

Issue Date
American Chemical Society
ACS Nano, Vol.8 No.9, pp.8887-8895
For the development of an intracellular cargo release system with mesoporous silica nanoparticles (MSN), gold nanoparticles coated with an amyloidogenic protein of alpha-synuclein were employed to prepare a protein-mediated nanocomposite into the "raspberry-type" particles-on-a-particle (PoP). The PoPs were successfully fabricated only at pH 4.4 by yielding the MSN coverage to 75.3% with 5 nm gold nanoparticles covalently coated with a mutant form of alpha-synuclein containing a cysteine residue at the C-terminus. The entrapped cargo of rhodamine 6G was shown to be selectively released from PoPs upon exposure to divalent cations including the alpha-synuclein-specific pathophysiological ligand of Ca2+. Intracellular uptake of the PoPs preloaded with doxorubicin as an anticancer drug and its subsequent Ca2+-dependent release were demonstrated with HeLa cells in the presence of intracellular Ca2+-regulating agents. Therefore, the fabrication of PoPs with the self-interactive protein of alpha-synuclein is expected to serve as a platform technology for preparation of diversified nanocomposites with various nanoparticles and/or bioactive molecules for eventual applications in the areas of theranostics.
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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