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Differentiation of actinomycete genera based on partial rpoB gene sequences
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Bum Joon | - |
dc.contributor.author | Koh, Young Hwan | - |
dc.contributor.author | Chun, Jong Sik | - |
dc.contributor.author | Kim, Chang Jin | - |
dc.contributor.author | Lee, Seung Hyun | - |
dc.contributor.author | Cho, Moon Jae | - |
dc.contributor.author | Hyun, Jin Won | - |
dc.contributor.author | Lee, Keun Hwa | - |
dc.contributor.author | Cha, Chang Yong | - |
dc.contributor.author | Kook, Yoon Hoh | - |
dc.date.accessioned | 2020-04-27T13:45:36Z | - |
dc.date.available | 2020-04-27T13:45:36Z | - |
dc.date.created | 2018-04-11 | - |
dc.date.issued | 2003-11 | - |
dc.identifier.citation | Journal of Microbiology and Biotechnology, Vol.13 No.6, pp.846-852 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.other | 32069 | - |
dc.identifier.uri | https://hdl.handle.net/10371/166119 | - |
dc.description.abstract | rpoB DNAs (279 bp) from 34 species of 5 actinomycete genera were sequenced and a phylogenetic tree was constructed based on the sequences obtained. The genera were clearly differentiated in the rpoB tree, forming clades specific to their respective genus. In addition, 2 signature amino acid residues specific to Streptomyces were found in a multiple alignment of the deduced amino acid sequences. To empirically confirm that this rpoB gene analysis system could be used to differentiate actinomycete isolates, the proposed system was used to identify 16 actinomycete isolates from Jeju Island. All isolates were successfully differentiated into the genera Streptomyces and Micromonospora. Accordingly, this is the first report that an rpoB sequence analysis has been effectively used to differentiate actinomycete strains at the genus level. | - |
dc.language | 영어 | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | Differentiation of actinomycete genera based on partial rpoB gene sequences | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 차창용 | - |
dc.contributor.AlternativeAuthor | 김범준 | - |
dc.contributor.AlternativeAuthor | 천종식 | - |
dc.contributor.AlternativeAuthor | 국윤호 | - |
dc.citation.journaltitle | Journal of Microbiology and Biotechnology | - |
dc.identifier.wosid | 000187661400003 | - |
dc.identifier.scopusid | 2-s2.0-0346097983 | - |
dc.citation.endpage | 852 | - |
dc.citation.number | 6 | - |
dc.citation.startpage | 846 | - |
dc.citation.volume | 13 | - |
dc.identifier.sci | 000187661400003 | - |
dc.identifier.kciid | ART001188443 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Bum Joon | - |
dc.contributor.affiliatedAuthor | Chun, Jong Sik | - |
dc.contributor.affiliatedAuthor | Cha, Chang Yong | - |
dc.contributor.affiliatedAuthor | Kook, Yoon Hoh | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | 16S RIBOSOMAL DNA | - |
dc.subject.keywordPlus | NUMERICAL CLASSIFICATION | - |
dc.subject.keywordPlus | STREPTOMYCES | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | MICROMONOSPORA | - |
dc.subject.keywordPlus | MYCOBACTERIUM | - |
dc.subject.keywordPlus | PHYLOGENY | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | FUNGI | - |
dc.subject.keywordAuthor | Phylogenetic relationship | - |
dc.subject.keywordAuthor | actinomycete genera | - |
dc.subject.keywordAuthor | rpoB | - |
dc.subject.keywordAuthor | signature amino acids | - |
dc.subject.keywordAuthor | differentiation | - |
dc.subject.keywordAuthor | identification | - |
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