Browse

MicroRNA genes are transcribed by RNA polymerase II

Cited 2680 time in Web of Science Cited 2894 time in Scopus
Authors
Lee, Yoontae; Kim, Minju; Han, Jin Ju; Yeom, Kyu‐Hyun; Lee, Sanghyuk; Baek, Sung Hee; Kim, V. Narry
Issue Date
2004-10
Citation
EMBO Journal, Vol.23 No.20, pp.4051-4060
Keywords
capDroshamicroRNApromotertranscription
Abstract
MicroRNAs (miRNAs) constitute a large family of noncoding RNAs that function as guide molecules in diverse gene silencing pathways. Current efforts are focused on the regulatory function of miRNAs, while little is known about how these unusual genes themselves are regulated. Here we present the first direct evidence that miRNA genes are transcribed by RNA polymerase II (pol II). The primary miRNA transcripts (pri-miRNAs) contain cap structures as well as poly(A) tails, which are the unique properties of class II gene transcripts. The treatment of human cells with alpha-amanitin decreased the level of pri-miRNAs at a concentration that selectively inhibits pol II activity. Furthermore, chromatin immunoprecipitation analyses show that pol II is physically associated with a miRNA promoter. We also describe, for the first time, the detailed structure of a miRNA gene by determining the promoter and the terminator of mir-23asimilar to27asimilar to24-2. These data indicate that pol II is the main, if not the only, RNA polymerase for miRNA gene transcription. Our study offers a basis for understanding the structure and regulation of miRNA genes.
ISSN
0261-4189
URI
https://hdl.handle.net/10371/171880
DOI
https://doi.org/10.1038/sj.emboj.7600385
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse