Browse

MicroRNA maturation: Stepwise processing and subcellular localization

Cited 1452 time in Web of Science Cited 1530 time in Scopus
Authors
Lee, Yoontae; Jeon, Kipyoung; Lee, Jun‐Tae; Kim, Sunyoung; Kim, V. Narry
Issue Date
2002-09
Citation
EMBO Journal, Vol.21 No.17, pp.4663-4670
Keywords
microRNAnuclear exportprocessingsubcellular localizationtranscription
Abstract
MicroRNAs (miRNAs) constitute a novel, phylogenetically extensive family of small RNAs (similar to22 nucleotides) with potential roles in gene regulation. Apart from the finding that miRNAs are produced by Dicer from the precursors of similar to70 nucleotides (pre-miRNAs), little is known about miRNA biogenesis. Some miRNA genes have been found in close conjunction, suggesting that they are expressed as single transcriptional units. Here, we present in vivo and in vitro evidence that these clustered miRNAs are expressed polycistronically and are processed through at least two sequential steps: (i) generation of the similar to70 nucleotide pre-miRNAs from the longer transcripts (termed pri-miRNAs); and (ii) processing of pre-miRNAs into mature miRNAs. Subcellular localization studies showed that the first and second steps are compartmentalized into the nucleus and cytoplasm, respectively, and that the pre-miRNA serves as the substrate for nuclear export. Our study suggests that the regulation of miRNA expression may occur at multiple levels, including the two processing steps and the nuclear export step. These data will provide a framework for further studies on miRNA biogenesis.
ISSN
0261-4189
URI
http://hdl.handle.net/10371/171882
DOI
https://doi.org/10.1093/emboj/cdf476
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse