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TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms
DC Field | Value | Language |
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dc.contributor.author | Kim, Boseon | - |
dc.contributor.author | Ha, Minju | - |
dc.contributor.author | Loeff, Luuk | - |
dc.contributor.author | Chang, Hyeshik | - |
dc.contributor.author | Simanshu, Dhirendra K. | - |
dc.contributor.author | Li, Sisi | - |
dc.contributor.author | Fareh, Mohamed | - |
dc.contributor.author | Patel, Dinshaw J. | - |
dc.contributor.author | Joo, Chirlmin | - |
dc.contributor.author | Kim, V. Narry | - |
dc.date.accessioned | 2021-01-31T08:11:47Z | - |
dc.date.available | 2021-01-31T08:11:47Z | - |
dc.date.created | 2018-10-30 | - |
dc.date.created | 2018-10-30 | - |
dc.date.issued | 2015-07 | - |
dc.identifier.citation | EMBO Journal, Vol.34 No.13, pp.1801-1815 | - |
dc.identifier.issn | 0261-4189 | - |
dc.identifier.other | 64287 | - |
dc.identifier.uri | https://hdl.handle.net/10371/171888 | - |
dc.description.abstract | Terminal uridylyl transferases (TUTs) function as integral regulators of microRNA (miRNA) biogenesis. Using biochemistry, single-molecule, and deep sequencing techniques, we here investigate the mechanism by which human TUT7 (also known as ZCCHC6) recognizes and uridylates precursor miRNAs (pre-miRNAs) in the absence of Lin28. We find that the overhang of a pre-miRNA is the key structural element that is recognized by TUT7 and its paralogues, TUT4 (ZCCHC11) and TUT2 (GLD2/PAPD4). For group II pre-miRNAs, which have a 1-nt 3' overhang, TUT7 restores the canonical end structure (2-nt 3' overhang) through mono-uridylation,thereby promoting miRNA biogenesis. For pre-miRNAs where the 3' end is further recessed into the stem (as in 3' trimmed pre-miRNAs), TUT7 generates an oligo-U tail that leads to degradation. In contrast to Lin28-stimulated oligo-uridylation, which is processive, a distributive mode is employed by TUT7 for both mono- and oligo-uridylation in the absence of Lin28. The overhang length dictates the frequency (but not duration) of the TUT7-RNA interaction, thus explaining how TUT7 differentiates pre-miRNA species with different overhangs. Our study reveals dual roles and mechanisms of uridylation in repair and removal of defective pre-miRNAs. | - |
dc.language | 영어 | - |
dc.publisher | Nature Publishing Group | - |
dc.title | TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김빛내리 | - |
dc.identifier.doi | 10.15252/embj.201590931 | - |
dc.citation.journaltitle | EMBO Journal | - |
dc.identifier.wosid | 000357324100008 | - |
dc.identifier.scopusid | 2-s2.0-84933670234 | - |
dc.citation.endpage | 1815 | - |
dc.citation.number | 13 | - |
dc.citation.startpage | 1801 | - |
dc.citation.volume | 34 | - |
dc.identifier.sci | 000357324100008 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, V. Narry | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | TERMINAL URIDYLYL TRANSFERASE | - |
dc.subject.keywordPlus | RNA-INTERFERENCE | - |
dc.subject.keywordPlus | NUCLEAR EXPORT | - |
dc.subject.keywordPlus | CYTOPLASMIC RNA | - |
dc.subject.keywordPlus | LET-7 MICRORNAS | - |
dc.subject.keywordPlus | PRE-MICRORNA | - |
dc.subject.keywordPlus | C-ELEGANS | - |
dc.subject.keywordPlus | BIOGENESIS | - |
dc.subject.keywordPlus | DICER | - |
dc.subject.keywordPlus | LIN28 | - |
dc.subject.keywordAuthor | precursor microRNA | - |
dc.subject.keywordAuthor | single-molecule fluorescence | - |
dc.subject.keywordAuthor | TUT4 (ZCCHC11) | - |
dc.subject.keywordAuthor | TUT7 (ZCCHC6) | - |
dc.subject.keywordAuthor | uridylation | - |
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