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Single-molecule approach to immunoprecipitated protein complexes: Insights into miRNA uridylation

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dc.contributor.authorYeom, Kyu-Hyeon-
dc.contributor.authorHeo, Inha-
dc.contributor.authorLee, Jinwoo-
dc.contributor.authorHohng, Sungchul-
dc.contributor.authorKim, V. Narry-
dc.contributor.authorJoo, Chirlmin-
dc.date.accessioned2021-01-31T08:11:54Z-
dc.date.available2021-01-31T08:11:54Z-
dc.date.issued2011-07-
dc.identifier.citationEMBO Reports, Vol.12 No.7, pp.690-696-
dc.identifier.issn1469-221X-
dc.identifier.other107039-
dc.identifier.urihttps://hdl.handle.net/10371/171890-
dc.description.abstractSingle-molecule techniques have been used for only a subset of biological problems because of difficulties in studying proteins that require cofactors or post-translational modifications. Here, we present a new method integrating single-molecule fluorescence microscopy and immunopurification to study protein complexes. We used this method to investigate Lin28-mediated microRNA uridylation by TUT4 (terminal uridylyl transferase 4, polyU polymerase), which regulates let-7 microRNA biogenesis. Our real-time analysis of the uridylation by the TUT4 immunoprecipitates suggests that Lin28 functions as a processivity factor of TUT4. Our new technique, SIMPlex (single-molecule approach to immunoprecipitated protein complexes), provides a universal tool to analyse complex proteins at the single-molecule level.-
dc.subjectsingle-molecule fluorescence-
dc.subjectsingle-molecule immunoprecipitation-
dc.subjectTUT4 (ZCCHC11)-
dc.subjectLin28 (Lin28a, Lin28b, Lin28)-
dc.subjectreal-time hybridization-
dc.titleSingle-molecule approach to immunoprecipitated protein complexes: Insights into miRNA uridylation-
dc.typeArticle-
dc.contributor.AlternativeAuthor김빛내리-
dc.identifier.doi10.1038/embor.2011.100-
dc.citation.journaltitleEMBO Reports-
dc.identifier.scopusid2-s2.0-79959936612-
dc.citation.endpage696-
dc.citation.number7-
dc.citation.startpage690-
dc.citation.volume12-
dc.identifier.urlhttps://www.embopress.org/doi/full/10.1038/embor.2011.100-
dc.identifier.rimsid107039-
dc.identifier.sci000292325700020-
dc.contributor.affiliatedAuthorKim, V. Narry-
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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