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Crystal structure of human DGCR8 core
DC Field | Value | Language |
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dc.contributor.author | Sohn, Sun Young | - |
dc.contributor.author | Bae, Won Jin | - |
dc.contributor.author | Kim, Jeong Joo | - |
dc.contributor.author | Yeom, Kyu-Hyeon | - |
dc.contributor.author | Kim, V. Narry | - |
dc.contributor.author | Cho, Yunje | - |
dc.date.accessioned | 2021-01-31T08:15:06Z | - |
dc.date.available | 2021-01-31T08:15:06Z | - |
dc.date.created | 2019-11-12 | - |
dc.date.issued | 2007-09 | - |
dc.identifier.citation | Nature Structural and Molecular Biology, Vol.14 No.9, pp.847-853 | - |
dc.identifier.issn | 1545-9993 | - |
dc.identifier.other | 85920 | - |
dc.identifier.uri | https://hdl.handle.net/10371/171945 | - |
dc.description.abstract | A complex of Drosha with DGCR8 ( or its homolog Pasha) cleaves primary microRNA ( pri- miRNA) substrates into precursor miRNA and initiates the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 or Pasha provides a frame for anchoring substrate pri- miRNAs. To clarify the molecular basis underlying recognition of pri- miRNA by DGCR8 and Pasha, we determined the crystal structure of the human DGCR8 core ( DGCR8S, residues 493 - 720). In the structure, the two double- stranded RNA - binding domains ( dsRBDs) are arranged with pseudo two- fold symmetry and are tightly packed against the C- terminal helix. The H2 helix in each dsRBD is important for recognition of pri- miRNA substrates. This structure, together with fluorescent resonance energy transfer and mutational analyses, suggests that the DGCR8 core recognizes pri- miRNA in two possible orientations. We propose a model for DGCR8' s recognition of pri- miRNA. | - |
dc.language | 영어 | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Crystal structure of human DGCR8 core | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김빛내리 | - |
dc.identifier.doi | 10.1038/nsmb1294 | - |
dc.citation.journaltitle | Nature Structural and Molecular Biology | - |
dc.identifier.wosid | 000249276500012 | - |
dc.identifier.scopusid | 2-s2.0-34548480185 | - |
dc.citation.endpage | 853 | - |
dc.citation.number | 9 | - |
dc.citation.startpage | 847 | - |
dc.citation.volume | 14 | - |
dc.identifier.sci | 000249276500012 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, V. Narry | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | RNA-BINDING DOMAIN | - |
dc.subject.keywordPlus | DIGEORGE-SYNDROME | - |
dc.subject.keywordPlus | MOLECULAR-BASIS | - |
dc.subject.keywordPlus | NUCLEAR EXPORT | - |
dc.subject.keywordPlus | MICROPROCESSOR COMPLEX | - |
dc.subject.keywordPlus | DROSHA-DGCR8 COMPLEX | - |
dc.subject.keywordPlus | PRIMARY MICRORNAS | - |
dc.subject.keywordPlus | ENZYME COMPLEX | - |
dc.subject.keywordPlus | C-ELEGANS | - |
dc.subject.keywordPlus | PROTEIN | - |
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