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The RNA-binding protein repertoire of embryonic stem cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, S. Chul | - |
dc.contributor.author | Yi, Hyerim | - |
dc.contributor.author | Eichelbaum, Katrin | - |
dc.contributor.author | Foehr, Sophia | - |
dc.contributor.author | Fischer, Bernd | - |
dc.contributor.author | You, Kwon Tae | - |
dc.contributor.author | Castello, Alfredo | - |
dc.contributor.author | Krijgsveld, Jeroen | - |
dc.contributor.author | Hentze, Matthias W. | - |
dc.contributor.author | Kim, V. Narry | - |
dc.date.accessioned | 2021-01-31T08:15:17Z | - |
dc.date.available | 2021-01-31T08:15:17Z | - |
dc.date.created | 2020-07-16 | - |
dc.date.created | 2020-07-16 | - |
dc.date.issued | 2013-09 | - |
dc.identifier.citation | Nature Structural and Molecular Biology, Vol.20 No.9, pp.1122-1130 | - |
dc.identifier.issn | 1545-9993 | - |
dc.identifier.other | 107061 | - |
dc.identifier.uri | https://hdl.handle.net/10371/171948 | - |
dc.description.abstract | RNA-binding proteins (RBPs) have essential roles in RNA-mediated gene regulation, and yet annotation of RBPs is limited mainly to those with known RNA-binding domains. To systematically identify the RBPs of embryonic stem cells (ESCs), we here employ interactome capture, which combines UV cross-linking of RBP to RNA in living cells, oligo(dT) capture and MS. From mouse ESCs (mESCs), we have defined 555 proteins constituting the mESC mRNA interactome, including 283 proteins not previously annotated as RBPs. Of these, 68 new RBP candidates are highly expressed in ESCs compared to differentiated cells, implicating a role in stem-cell physiology. Two well-known E3 ubiquitin ligases, Trim25 (also called Efp) and Trim71 (also called Lin41), are validated as RBPs, revealing a potential link between RNA biology and protein-modification pathways. Our study confirms and expands the atlas of RBPs, providing a useful resource for the study of the RNA-RBP network in stem cells. | - |
dc.language | 영어 | - |
dc.publisher | Nature Publishing Group | - |
dc.title | The RNA-binding protein repertoire of embryonic stem cells | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김빛내리 | - |
dc.identifier.doi | 10.1038/nsmb.2638 | - |
dc.citation.journaltitle | Nature Structural and Molecular Biology | - |
dc.identifier.wosid | 000324160900017 | - |
dc.identifier.scopusid | 2-s2.0-84883741725 | - |
dc.citation.endpage | 1130 | - |
dc.citation.number | 9 | - |
dc.citation.startpage | 1122 | - |
dc.citation.volume | 20 | - |
dc.identifier.sci | 000324160900017 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, V. Narry | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | RESPONSIVE FINGER PROTEIN | - |
dc.subject.keywordPlus | COSTIMULATOR MESSENGER-RNA | - |
dc.subject.keywordPlus | E3 UBIQUITIN LIGASE | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL COACTIVATOR | - |
dc.subject.keywordPlus | NUCLEOTIDE-RESOLUTION | - |
dc.subject.keywordPlus | LET-7 MICRORNA | - |
dc.subject.keywordPlus | SOMATIC-CELLS | - |
dc.subject.keywordPlus | TARGET GENE | - |
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