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Molecular basis for the single-nucleotide precision of primary microRNA processing
DC Field | Value | Language |
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dc.contributor.author | Kwon, S. Chul | - |
dc.contributor.author | Baek, S. Chan | - |
dc.contributor.author | Choi, Yeon-Gil | - |
dc.contributor.author | Yang, Jihye | - |
dc.contributor.author | Lee, Young-suk | - |
dc.contributor.author | Woo, Jae-Sung | - |
dc.contributor.author | Kim, V. Narry | - |
dc.date.accessioned | 2021-01-31T08:15:20Z | - |
dc.date.available | 2021-01-31T08:15:20Z | - |
dc.date.created | 2019-08-23 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | Molecular Cell, Vol.73 No.3, pp.505-518.e5 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.other | 81808 | - |
dc.identifier.uri | https://hdl.handle.net/10371/171949 | - |
dc.description.abstract | Microprocessor, composed of DROSHA and its cofactor DGCR8, initiates microRNA(miRNA) biogenesis by processing the primary transcripts of miRNA (pri-miRNAs). Here we investigate the mechanism by which Microprocessor selects the cleavage site with single-nucleotide precision, which is crucial for the specificity and functionality of miRNAs. By testing similar to 40,000 pri-miRNA variants, we find that for some pri-miRNAs the cleavage site is dictated mainly by the mGHG motif embedded in the lower stem region of pri-miRNA. Structural modeling and deep-sequencing-based complementation experiments show that the double-stranded RNA-binding domain (dsRBD) of DROSHA recognizes mGHG to place the catalytic center in the appropriate position. The mGHG motif as well as the mGHG-recognizing residues in DROSHA dsRBD are conserved across eumetazoans, suggesting that this mechanism emerged in an early ancestor of the animal lineage. Our findings provide a basis for the understanding of miRNA biogenesis and rational design of accurate small-RNA-based gene silencing. | - |
dc.language | 영어 | - |
dc.publisher | Cell Press | - |
dc.title | Molecular basis for the single-nucleotide precision of primary microRNA processing | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김빛내리 | - |
dc.identifier.doi | 10.1016/j.molcel.2018.11.005 | - |
dc.citation.journaltitle | Molecular Cell | - |
dc.identifier.wosid | 000458015200011 | - |
dc.identifier.scopusid | 2-s2.0-85061010954 | - |
dc.citation.endpage | 518.e5 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 505 | - |
dc.citation.volume | 73 | - |
dc.identifier.sci | 000458015200011 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, V. Narry | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | RNA-INTERFERENCE | - |
dc.subject.keywordPlus | RIBONUCLEASE-III | - |
dc.subject.keywordPlus | SEQUENCE DETERMINANTS | - |
dc.subject.keywordPlus | READ ALIGNMENT | - |
dc.subject.keywordPlus | DICER | - |
dc.subject.keywordPlus | DROSHA | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | PRECURSORS | - |
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